Expression of proteins related to thyroid hormone function in the uterus is down‐regulated at the day of implantation in hypothyroid pregnant rats

Autor: Naguib Salleh, Nelli Giribabu, Abu Sadat Md Sayem, Si Lay Khaing
Rok vydání: 2019
Předmět:
0301 basic medicine
Infertility
Thyroid Hormones
endocrine system
medicine.medical_specialty
endocrine system diseases
MAP Kinase Signaling System
Receptors
Retinoic Acid
Thyroid Gland
Uterus
Down-Regulation
Rats
Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Hypothyroidism
Pregnancy
Internal medicine
Animals
Medicine
Euthyroid
Embryo Implantation
Methimazole
Receptors
Thyroid Hormone
Thyroid hormone receptor
business.industry
Thyroid
Gene Expression Regulation
Developmental
Receptors
Thyrotropin
Uterine horns
Cell Biology
General Medicine
medicine.disease
Rats
Thyroxine
Retinoic acid receptor
030104 developmental biology
medicine.anatomical_structure
Endocrinology
030220 oncology & carcinogenesis
Female
business
hormones
hormone substitutes
and hormone antagonists
Hormone
Zdroj: Cell Biology International. 43:486-494
ISSN: 1095-8355
1065-6995
Popis: Hypothyroidism has been linked to infertility, but the mechanisms underlying infertility-related hypothyroidism have yet to be fully elucidated. Therefore, in this study, effects of hypothyroidism on expression of the proteins related to thyroid hormone function in the uterus, which were thought to play a role implantation, including thyroid hormone receptor (TR), thyroid stimulating hormone receptor (TSHR), retinoic acid receptor (RAR) and extracellular kinase (ERK) were identified. Pregnant female rats were rendered hypothyroid by giving methimazole (MMI), orally. Following hypothyroid induction, rats were grouped into control (non-treated) and received subcutaneous thyroxine at 20, 40, and 80 μg/kg/day for five consecutive days. At Day 6, which is the day of implantation (GD 6), rats were sacrificed and the number of embryo implantation site in the uterus was calculated. Then, uterine horns were harvested and expression of the above proteins and their mRNAs were identified by Western blotting and real-time PCR, respectively. In non-treated hypothyroid pregnant rats, the number of embryo implantation sites decreased as compared to euthyroid and hypothyroid rats receiving thyroxine treatment. Similarly, expression of TRα-1, TRβ-1, TSHR, ERK1/2 and RAR proteins and mRNA in the uterus of non-treated hypothyroid rats also decreased (P < 0.05 when compared to euthyroid and thyroxine-treated hypothyroid rats). In conclusion, downregulated expression of the thyroid hormone related proteins in the uterus at the day of implantation might result in infertility as reported in hypothyroid condition.
Databáze: OpenAIRE
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