Effect of Microenvironment on Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Hepatocytes In Vitro and In Vivo
| Autor: | Yabin Qin, Honghai Wu, Yanning Hou, Yang Hong, Jianfang Liu, Xiaolei Han, Xin Ma, Gai Xue, Yu-Qin Hu |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Article Subject Cellular differentiation Cell lcsh:Medicine Cord Blood Stem Cell Transplantation Mesenchymal Stem Cell Transplantation General Biochemistry Genetics and Molecular Biology Umbilical Cord Cell therapy 03 medical and health sciences In vivo medicine Animals Humans Carbon Tetrachloride General Immunology and Microbiology business.industry Mesenchymal stem cell lcsh:R Cell Differentiation Mesenchymal Stem Cells General Medicine In vitro Rats Transplantation 030104 developmental biology medicine.anatomical_structure Liver Cancer research Hepatocytes Chemical and Drug Induced Liver Injury business Biomarkers Research Article |
| Zdroj: | BioMed Research International, Vol 2016 (2016) BioMed Research International |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | Human umbilical cord-derived mesenchymal stem cells (hUCMSCs) are considered to be an ideal cell source for cell therapy of many diseases. The aim of this study was to investigate the contribution of the microenvironment to the hepatic differentiation potential of hUCMSCsin vitroandin vivoand to explore their therapeutic use in acute liver injury in rats. We established a new model to simulate the liver tissue microenvironmentin vivousing liver homogenate supernatant (LHS)in vitro. This induced environment could drive hUCMSCs to differentiate into hepatocyte-like cells within 7 days. The differentiated cells expressed hepatocyte-specific markers and demonstrated hepatocellular functions. We also injected hUCMSCs into rats with CCl4-induced acute hepatic injury. The hUCMSCs were detected in the livers of recipient rats and expressed the human hepatocyte-specific markers, suggesting that hUCMSCs could differentiate into hepatocyte-like cellsin vivoin the liver tissue microenvironment. Levels of biochemistry markers improved significantly after transplantation of hUCMSCs compared with the nontransplantation group (P<0.05). In conclusion, this study demonstrated that the liver tissue microenvironment may contribute to the differentiation of hUCMSCs into hepatocytes bothin vitroandin vivo. |
| Databáze: | OpenAIRE |
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