CXCR5+CD4+ follicular helper T cells accumulate in resting human lymph nodes and have superior B cell helper activity
| Autor: | Frederike J. Bemelman, Ester B. M. Remmerswaal, René A. W. van Lier, Karlijn A M I van der Pant, Mirza M. Idu, Nelly van der Bom, Ineke J. M. ten Berge, Simone H. C. Havenith, Ester M. M. van Leeuwen |
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| Přispěvatelé: | Other departments, Amsterdam institute for Infection and Immunity, Experimental Immunology, Amsterdam Cardiovascular Sciences, Surgery, Nephrology, Landsteiner Laboratory |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Adult
Cytotoxicity Immunologic Graft Rejection Receptors CXCR5 Helper T lymphocyte Immunology Cell Communication Biology Immunophenotyping Young Adult Immune system Antigen T-Lymphocyte Subsets medicine Immunology and Allergy Cytotoxic T cell Humans Lymph node B cell Cells Cultured Aged B-Lymphocytes Blood Cells General Medicine T lymphocyte T-Lymphocytes Helper-Inducer Middle Aged Kidney Transplantation medicine.anatomical_structure CD4 Antigens Transplantation Tolerance Lymph Lymph Nodes Immunologic Memory |
| Zdroj: | International immunology, 26(3), 183-192. Oxford University Press |
| ISSN: | 0953-8178 |
| Popis: | Although many relevant immune reactions are initiated in the lymph nodes, this compartment has not been systematically studied in humans. Analyses have been performed on immune cells derived from tonsils, but as this tissue is most often inflamed, generalization of these data is difficult. Here, we analyzed the phenotype and function of the human CD4+ T-cell subsets and lineages in paired resting lymph node and peripheral blood samples. Naive, central memory cells and effector memory cells as well as Th1, Th2, Th17 and Treg cells were equally represented in both compartments. On the other hand, cytotoxic CD4+ T cells were strikingly absent in the lymph nodes. CXCR5+CD4+ T cells, representing putative follicular Th (Tfh) cells were over-represented in lymph nodes and expressed higher levels of Tfh markers than their peripheral blood counterparts. Compared with the circulating pool, lymph-node-derived CXCR5+CD4+ T cells were superior in providing help to B cells. Thus, functionally competent Tfh cells accumulate in resting human lymph nodes, providing a swift induction of naive and memory antibody responses upon antigenic challenge. |
| Databáze: | OpenAIRE |
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