Inhibition of thrombin, an unexplored function of retinoic acid
Autor: | Chelliah Jayabaskaran, K. Akhilender Naidu, Maheswaraiah Anikisetty, Tirumala Harikrishna Anantha Krishna, William R Surin, Subban Kamalraj |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Vitamin PRP platelet rich plasma Biophysics Retinoic acid PE pigment epithelium Pharmacology Biochemistry lcsh:Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Thrombin In vivo medicine Thrombin inhibition lcsh:QD415-436 AFU arbitrary fluorescence units lcsh:QH301-705.5 chemistry.chemical_classification Retinol In vitro ADP adenosine diphosphate 030104 developmental biology Enzyme chemistry lcsh:Biology (General) 030220 oncology & carcinogenesis Retinaldehyde Platelet aggregation inhibition AMC 7-amino 4-methyl coumarin RBP retinol binding protein medicine.drug Research Article circulatory and respiratory physiology |
Zdroj: | Biochemistry and Biophysics Reports, Vol 18, Iss, Pp-(2019) Biochemistry and Biophysics Reports |
ISSN: | 2405-5808 |
Popis: | Retinoic acid, a derivative of vitamin A, is known to possess in vivo anti-inflammatory, anti-platelet and fibrinolytic activities. We have investigated the in vitro thrombin and platelet aggregation inhibitory activities of vitamin A (retinol) and its derivatives, retinoic acid and retinaldehyde. The thrombin enzymatic assay was performed fluorimetrically to assess the inhibition of thrombin (Sigma and plasma). Retinoic acid, retinaldehyde and retinol exhibited potent inhibition of thrombin, with IC50 values of 67μg/ml, 74μg/ml and 152μg/ml, respectively for the inhibition of thrombin (Sigma); and 49μg/ml, 74μg/ml and 178μg/ml, respectively for the inhibition of thrombin (plasma). Amongst vitamin A and its derivatives, retinoic acid showed the highest inhibition of both the forms of thrombin. Vitamin A and its derivatives also displayed remarkable inhibition of platelet aggregation. This is the first report of vitamin A and its derivatives showing inhibition of thrombin and platelet aggregation in vitro. Highlights • Inhibition of thrombin by retinoic acid in vitro. • Comparison of in vitro thrombin inhibitory activity of retinoic acid with retinaldehyde and retinol. • Inhibition of platelet aggregation by vitamin A and its derivatives in vitro. |
Databáze: | OpenAIRE |
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