An Intact Kidney Slice Model to Investigate Vasa Recta Properties and Function in situ

Autor: L. Sawbridge, J. Munday, Carol Crawford, Teresa M Kennedy-Lydon, Claire M. Peppiatt-Wildman, Tejal A. Desai, Robert J. Unwin, C. Sprott, Scott S.P. Wildman
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Male
Sympathetic Nervous System
Physiology
Indomethacin
030204 cardiovascular system & hematology
Kidney
law.invention
Rats
Sprague-Dawley
chemistry.chemical_compound
Norepinephrine
0302 clinical medicine
Adenosine Triphosphate
Microvasculature
law
Vasoconstrictor Agents
Enzyme Inhibitors
0303 health sciences
Kidney Medulla
Microscopy
Confocal
Endothelin-1
Innervation
Angiotensin II
General Medicine
Anatomy
Medulla
Immunohistochemistry
Cell biology
medicine.anatomical_structure
NG-Nitroarginine Methyl Ester
Nephrology
cardiovascular system
Proteoglycans
medicine.symptom
Tissue slicing
Signal Transduction
RM
Cell Survival
Biology
In Vitro Techniques
Tubulovascular crosstalk
03 medical and health sciences
Confocal microscopy
Physiology (medical)
medicine
Animals
Propidium iodide
Antigens
030304 developmental biology
Original Paper
urogenital system
fungi
Hemodynamics
Vasa recta
Live kidney slice model
Capillaries
Rats
Calcein
chemistry
Vasoconstriction
Pericytes
Zdroj: Nephron. Physiology
ISSN: 1660-2137
Popis: Background: Medullary blood flow is via vasa recta capillaries, which possess contractile pericytes. In vitro studies using isolated descending vasa recta show that pericytes can constrict/dilate descending vasa recta when vasoactive substances are present. We describe a live kidney slice model in which pericyte-mediated vasa recta constriction/dilation can be visualized in situ. Methods: Confocal microscopy was used to image calcein, propidium iodide and Hoechst labelling in ‘live’ kidney slices, to determine tubular and vascular cell viability and morphology. DIC video-imaging of live kidney slices was employed to investigate pericyte-mediated real-time changes in vasa recta diameter. Results: Pericytes were identified on vasa recta and their morphology and density were characterized in the medulla. Pericyte-mediated changes in vasa recta diameter (10–30%) were evoked in response to bath application of vasoactive agents (norepinephrine, endothelin-1, angiotensin-II and prostaglandin E2) or by manipulating endogenous vasoactive signalling pathways (using tyramine, L-NAME, a cyclo-oxygenase (COX-1) inhibitor indomethacin, and ATP release). Conclusions: The live kidney slice model is a valid complementary technique for investigating vasa recta function in situ and the role of pericytes as regulators of vasa recta diameter. This technique may also be useful in exploring the role of tubulovascular crosstalk in regulation of medullary blood flow.
Databáze: OpenAIRE
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