Iron uptake by Caco-2 cells following in vitro digestion: Effects of heat treatments of pork meat and pH of the digests
| Autor: | A. D. Sørensen, K. Bukhave |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Hot Temperature
Meat Swine Iron Biochemistry Inorganic Chemistry fluids and secretions Pepsin medicine Animals Humans Food science Iron uptake biology Chemistry food and beverages Biological Transport Hydrogen-Ion Concentration Ascorbic acid Pepsin A In vitro medicine.anatomical_structure Caco-2 Cell culture Pancreatin Pork meat Duodenum biology.protein Molecular Medicine Caco-2 Cells |
| Zdroj: | Journal of Trace Elements in Medicine and Biology. 24:230-235 |
| ISSN: | 0946-672X |
| Popis: | The present in vitro studies report on iron uptake by Caco-2 cells from pepsin and pepsin+pancreatin-digested pork meat proteins at pH values between 4.6 and 7 mimicking conditions in the duodenum and the proximal jejunum, respectively. Heat treatment of the pork meat resulted in increased iron uptake from pepsin-digested samples to Caco-2 cells at pH 4.6. The major enhancing effects on iron uptake by Caco-2 cells were observed after pepsin digestion in the pH range 4.6-6.0, whereas the pepsin+pancreatin-digested samples resulted in negligible iron uptake in Caco-2 cells at pH 7. Thus, the results emphasize the importance of separating pepsin-digested and pepsin+pancreatin-digested proteins during in vitro studies on iron availability. Furthermore, the present results showed the pH dependency of iron uptake anticipated. The enhancing effect of ascorbic acid was verified by increased iron uptake from pepsin-digested pork meat samples at pH 4.6, while no effect of ascorbic acid was observed at pH 7 in pepsin+pancreatin-digested samples. |
| Databáze: | OpenAIRE |
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