Resourcing, annotating, and analysing synthetic peptides of SARS-CoV-2 for immunopeptidomics and other immunological studies
| Autor: | Jerico Revote, Nicole A. Mifsud, Rochelle Ayala, Katherine E. Scull, Asolina Braun, Nathan P. Croft, Pouya Faridi, Sri H. Ramarathinam, Chen Li, Anthony W. Purcell, Shan Zou Chung, Ziyi Huang, Patricia T. Illing |
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| Rok vydání: | 2021 |
| Předmět: |
Proteomics
COVID19 T cell Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Peptide Human leukocyte antigen Computational biology Biology Tandem mass spectrometry Biochemistry LC–MS SARS‐CoV‐2 03 medical and health sciences Immune system Tandem Mass Spectrometry medicine Humans Spectral data Molecular Biology Research Articles database 030304 developmental biology chemistry.chemical_classification 0303 health sciences SARS-CoV-2 030302 biochemistry & molecular biology COVID-19 medicine.anatomical_structure chemistry Proteome synthetic peptides Peptides Research Article |
| Zdroj: | Proteomics |
| ISSN: | 1615-9861 |
| Popis: | SARS‐CoV‐2 has caused a significant ongoing pandemic worldwide. A number of studies have examined the T cell mediated immune responses against SARS‐CoV‐2, identifying potential T cell epitopes derived from the SARS‐CoV‐2 proteome. Such studies will aid in identifying targets for vaccination and immune monitoring. In this study, we applied tandem mass spectrometry and proteomic techniques to a library of ∼40,000 synthetic peptides, in order to generate a large dataset of SARS‐CoV‐2 derived peptide MS/MS spectra. On this basis, we built an online knowledgebase, termed virusMS (https://virusms.erc.monash.edu/), to document, annotate and analyse these synthetic peptides and their spectral information. VirusMS incorporates a user‐friendly interface to facilitate searching, browsing and downloading the database content. Detailed annotations of the peptides, including experimental information, peptide modifications, predicted peptide‐HLA (human leukocyte antigen) binding affinities, and peptide MS/MS spectral data, are provided in virusMS. |
| Databáze: | OpenAIRE |
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