A high-frequency single nucleotide polymorphism in the MtrB sensor kinase in clinical strains of Mycobacterium tuberculosis alters its biochemical and physiological properties
| Autor: | Krishna Kumar Singh, M. S. Krishna, Vandana Malhotra, Uchenna Watson Waturuocha, Deepak Kumar Saini, P J Athira |
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| Rok vydání: | 2021 |
| Předmět: |
Single Nucleotide Polymorphisms
Cell Membranes Gene Expression medicine.disease_cause Biochemistry Database and Informatics Methods Gene expression Post-Translational Modification Phosphorylation Regulation of gene expression Genetics Mutation Multidisciplinary Virulence Kinase RNA-Binding Proteins Enzymes Actinobacteria Medicine Cellular Structures and Organelles Signal transduction Signal Transduction Research Article Science Single-nucleotide polymorphism Biology Research and Analysis Methods Polymorphism Single Nucleotide Mycobacterium tuberculosis Bacterial Proteins DNA-binding proteins medicine Humans Tuberculosis Bacteria Organisms Phosphatases Biology and Life Sciences Proteins Gene Expression Regulation Bacterial Cell Biology biology.organism_classification Biological Databases Mutation Databases Enzymology Transcription Factors |
| Zdroj: | PLoS ONE PLoS ONE, Vol 16, Iss 9, p e0256664 (2021) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0256664 |
| Popis: | The DNA polymorphisms found in clinical strains ofMycobacterium tuberculosisdrive altered physiology, virulence, and pathogenesis in them. Although the lineages of these clinical strains can be traced back to common ancestor/s, there exists a plethora of difference between them, compared to those that have evolved in the laboratory. We identify a mutation present in ~80% of clinical strains, which maps in the HATPase domain of the sensor kinase MtrB and alters kinase and phosphatase activities, and affects its physiological role. The changes conferred by the mutation were probed byin-vitrobiochemical assays which revealed changes in signaling properties of the sensor kinase. These changes also affect bacterial cell division rates, size and membrane properties. The study highlights the impact of DNA polymorphisms on the pathophysiology of clinical strains and provides insights into underlying mechanisms that drive signal transduction in pathogenic bacteria. |
| Databáze: | OpenAIRE |
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