Effects of different peep levels on mesenteric leukocyte-endothelial interactions in rats during mechanical ventilation
| Autor: | Rogério Pazetti, Naomi Kondo Nakagawa, Paulina Sannomiya, Priscila Aikawa, Thais Mauad, Maria Aparecida Oliveira, Sandra Helena Poliselli Farsky |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Male
Respiratory rate medicine.medical_treatment Clinical Sciences Blood Pressure Positive-Pressure Respiration Random Allocation Mechanical ventilation Leukocyte-endothelial interactions Leukocytes Animals Medicine Mesentery Splanchnic Circulation Rats Wistar Positive end-expiratory pressure Tidal volume Analysis of Variance lcsh:R5-920 business.industry Microcirculation General Medicine Blood flow Oxygenation respiratory system medicine.disease Rats respiratory tract diseases Blood pressure Mesenteric ischemia Anesthesia Models Animal Endothelium Vascular business lcsh:Medicine (General) therapeutics circulatory and respiratory physiology |
| Zdroj: | Clinics, Vol 64, Iss 5, Pp 443-450 (2009) Clinics, Volume: 64, Issue: 5, Pages: 443-450, Published: MAY 2009 Clinics (Sao Paulo, Brazil) Clinics; v. 64 n. 5 (2009); 443-450 Clinics; Vol. 64 Núm. 5 (2009); 443-450 Clinics; Vol. 64 No. 5 (2009); 443-450 Clinics Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1980-5322 1807-5932 |
| Popis: | INTRODUCTION: Mechanical ventilation with positive end expiratory pressure (PEEP) improves oxygenation and treats acute pulmonary failure. However, increased intrathoracic pressure may cause regional blood flow alterations that may contribute to mesenteric ischemia and gastrointestinal failure. We investigated the effects of different PEEP levels on mesenteric leukocyte-endothelial interactions. METHODS: Forty-four male Wistar rats were initially anesthetized (Pentobarbital I.P. 50mg/kg) and randomly assigned to one of the following groups: 1) NAIVE (only anesthesia; n=9), 2) PEEP 0 (PEEP of 0 cmH2O, n=13), 3) PEEP 5 (PEEP of 5 cmH2O, n=12), and 4) PEEP 10 (PEEP of 10 cmH2O, n=13). Positive end expiratory pressure groups were tracheostomized and mechanically ventilated with a tidal volume of 10 mL/kg, respiratory rate of 70 rpm, and inspired oxygen fraction of 1. Animals were maintained under isoflurane anesthesia. After two hours, laparotomy was performed, and leukocyte-endothelial interactions were evaluated by intravital microscopy. RESULTS: No significant changes were observed in mean arterial blood pressure among groups during the study. Tracheal peak pressure was smaller in PEEP 5 compared with PEEP 0 and PEEP 10 groups (11, 15, and 16 cmH2O, respectively; p |
| Databáze: | OpenAIRE |
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