Separation and evaluation of natural antileishmanial potential against Leishmania major and infuntum isolated from the Tunisia strains
| Autor: | Mossadok Ben Attia, Safia El-Bok, Sawssen Selmi, Salwa Bouabdallah, Nabiha Bouzouita, Dhafer Laouini, Rabiaa M. Sghaier |
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| Rok vydání: | 2018 |
| Předmět: |
Pharmacology
chemistry.chemical_classification High peak Tribulus terrestris Tunisia Chloroform biology Tribulus Traditional medicine Chemistry lcsh:RM1-950 Saponin biology.organism_classification In vitro Leishmania infuntum chemistry.chemical_compound lcsh:Therapeutics. Pharmacology Leishmania major MTT assay Antileishmanial |
| Zdroj: | Bangladesh Journal of Pharmacology, Vol 13, Iss 1 (2018) |
| ISSN: | 1991-0088 |
| DOI: | 10.3329/bjp.v13i1.33908 |
| Popis: | Besides its nutritional value as a dietary supplement, Tribulus terrestris is used as a remedy for fertility disorder in Ayurveda and Chinese medicine as well as by modern herbalists. The aim of this study was to explore the biological potential (antileishmanial effect) of an extract rich in saponins from Tunisian tribulus. The chloroform extract of the various parts of T. terrestris was subjected to partial purification by solvent partitioning with ethanol and n-butanol. All prepared extracts were tested for their anti-leishmanial activity. The result showed that n-butanolic extract (saponin fraction, when isolated from leaves part) exhibited the best antileishmanial effect against both pathogenic parasites Leshmania L. major (GlC94) and L. infuntum (LV50) evaluated in vitro assessment through MTT assay. n-Butanolic extract had been detected, quantified and purified using the RP-HPLC finger print (Hypersil ODS coupled to UV-vis). High peak area (5116.82 at 3.03 min) was detected at 205 nm. |
| Databáze: | OpenAIRE |
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