Elevation of Bombina variegata peptide 8 in mice with collagen-induced arthritis
| Autor: | Saburo Saito, Ken Yoshida, Maimi Yanagimachi, Eigo Takahashi, Daitaro Kurosaka, Taro Ukichi, Kazuhiro Furuya, Akio Yamada, Kentaro Noda, Isamu Kingetsu |
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| Rok vydání: | 2009 |
| Předmět: |
Male
musculoskeletal diseases Pathology medicine.medical_specialty lcsh:Diseases of the musculoskeletal system Arthritis Bombina variegata Gastrointestinal Hormones Pathogenesis Mice Rheumatology Downregulation and upregulation Bone Marrow Internal medicine medicine Animals Orthopedics and Sports Medicine RNA Messenger biology Reverse Transcriptase Polymerase Chain Reaction business.industry Neuropeptides Synovial Membrane musculoskeletal system medicine.disease biology.organism_classification Arthritis Experimental Up-Regulation Disease Models Animal medicine.anatomical_structure Mice Inbred DBA biology.protein Joints Collagen Bone marrow lcsh:RC925-935 Antibody Synovial membrane business Biomarkers Research Article |
| Zdroj: | BMC Musculoskeletal Disorders, Vol 10, Iss 1, p 45 (2009) BMC Musculoskeletal Disorders |
| ISSN: | 1471-2474 |
| DOI: | 10.1186/1471-2474-10-45 |
| Popis: | Background Bombina variegate peptide 8 (Bv8) is a small protein secreted by frog skin. Recently it has been shown to contribute to tumor angiogenesis in mouse model. The purpose of this study was to investigate Bv8 in mice with type II collagen-induced arthritis (CIA). Methods We induced CIA in male DBA/1J mice. The severity of arthritis was evaluated based on an arthritis score. RNA was extracted from the joint, and examined for Bv8 mRNA expression by RT-PCR and real-time RT-PCR. Synovial tissue and bone marrow were immunohistochemically examined using anti-Bv8 antibody. Results The level of Bv8 mRNA expression in the joint was below the detection limit in the control group, but was elevated in the CIA group, and was correlated with the arthritis score. In addition, an increase in Bv8-positive cells was observed in the synovium and bone marrow in the CIA group. Conclusion Bv8 was elevated in the synovium and bone marrow of CIA mice, suggesting that Bv8 plays an important role in the pathogenesis of arthritis. |
| Databáze: | OpenAIRE |
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