Correlation of hormone receptor status between circulating tumor cells, primary tumor, and metastasis in breast cancer patients
| Autor: | Karina Wong, Matthew Maurer, Eligie Villarin, Julie Ann Mayer, Tony J. Pircher, Xunhai Xu, Farideh Z. Bischoff, Tam Pham, Mindy Brown, Kevin Kalinsky |
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| Rok vydání: | 2014 |
| Předmět: |
Oncology
CA15-3 Adult Cancer Research medicine.medical_specialty Lung Neoplasms Estrogen receptor CA 15-3 Bone Neoplasms Breast Neoplasms Soft Tissue Neoplasms Article Metastasis Cohort Studies Tumor Status Breast cancer Circulating tumor cell Internal medicine Cell Line Tumor Biomarkers Tumor Medicine Humans Prospective Studies skin and connective tissue diseases Thoracic Wall Aged business.industry Brain Neoplasms Liver Neoplasms General Medicine Middle Aged medicine.disease Neoplastic Cells Circulating Primary tumor Immunohistochemistry Receptors Estrogen MCF-7 Cells Female Lymph Nodes business Receptors Progesterone |
| Zdroj: | Clinicaltranslational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico. 17(7) |
| ISSN: | 1699-3055 |
| Popis: | Estrogen receptor (ER) and progesterone receptor (PR) status is prognostic and predictive in breast cancer. Because metastatic breast tumor biopsies are not routinely feasible, circulating tumor cells (CTCs) offer an alternative source of determining ER/PR tumor status.Peripheral blood was collected prospectively from 36 patients with metastatic breast cancer. CTCs were isolated using the microfluidic OncoCEE™ platform. Detection was accomplished with an expanded anti-cytokeratin (CK) cocktail mixture and anti-CD45. ER/PR protein expression was assessed by immunocytochemistry (ICC) on the CK+ cells and compared to the primary and/or metastatic tumor by immunohistochemistry (IHC).Among the 24 CK + CTC cases, a concordance of 68 % (15/22) in ER/PR status between primary breast tumor and CTCs and 83 % (10/12) between metastatic tumor and CTCs was observed. An overall concordance of 79 % (19/24) was achieved when assessing CTC and metastatic tumor (primary tumor substituted if metastatic breast biopsy not available). A test sensitivity of 72 % and specificity of 100 % was identified when comparing CTCs to tumor tissue. Of the 7 discordant cases between CTCs and primary tumor tissue, 2 were concordant with the metastatic biopsy.CTC ER/PR status using the OncoCEE™ platform is feasible, with high concordance in ER/PR status between tumor tissue (IHC) and CTCs (ICC). The prognostic and predictive significance of CTC ER/PR protein expression needs further evaluation in larger trials. |
| Databáze: | OpenAIRE |
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