Fresh ginger (Zingiber officinale) has anti-viral activity against human respiratory syncytial virus in human respiratory tract cell lines
| Autor: | Kuo Chih Wang, Den En Shieh, Chia Feng Yeh, Jung San Chang, Lien Chai Chiang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Cell Survival
Respiratory System Microbial Sensitivity Tests Viral Plaque Assay Ginger Biology Antiviral Agents Virus Cell Line Microbiology Drug Discovery medicine Humans Respiratory system Pharmacology A549 cell Virus quantification Dose-Response Relationship Drug Plant Extracts Tumor Necrosis Factor-alpha Water Interferon-beta medicine.anatomical_structure Cell culture Respiratory Syncytial Virus Human Respiratory epithelium Zingiber officinale Drugs Chinese Herbal Respiratory tract |
| Zdroj: | Journal of Ethnopharmacology. 145:146-151 |
| ISSN: | 0378-8741 |
| DOI: | 10.1016/j.jep.2012.10.043 |
| Popis: | Ginger, Zingiber officinale Roscoe, is a common spice and also a widely used medicinal plant in ancient China. Ginger is an ingredient of Ge-Gen-Tang (Kakkon-to; GGT). GGT has been proved to have antiviral activity against human respiratory syncytial virus (HRSV). However, it is unknown whether ginger is effective against HRSV.To find a readily available agent to manage HRSV infection, the authors tested the hypothesis that ginger can effectively decrease HRSV-induced plaque formation in respiratory mucosal cell lines.Effect of hot water extracts of fresh and dried gingers on HRSV was tested by plaque reduction assay in both human upper (HEp-2) and low (A549) respiratory tract cell lines. Ability of ginger to stimulate anti-viral cytokines was evaluated by enzyme-linked immunosorbent assay (ELISA).Fresh ginger dose-dependently inhibited HRSV-induced plaque formation in both HEp-2 and A549 cell lines (p0.0001). In contrast, dried ginger didn't show any dose-dependent inhibition. 300 μg/ml fresh ginger could decrease the plaque counts to 19.7% (A549) and 27.0% (HEp-2) of that of the control group. Fresh ginger was more effective when given before viral inoculation (p0.0001), particularly on A549 cells. 300 μg/ml fresh ginger could decrease the plaque formation to 12.9% when given before viral inoculation. Fresh ginger dose-dependently inhibited viral attachment (p0.0001) and internalization (p0.0001). Fresh ginger of high concentration could stimulate mucosal cells to secrete IFN-β that possibly contributed to counteracting viral infection.Fresh, but not dried, ginger is effective against HRSV-induced plaque formation on airway epithelium by blocking viral attachment and internalization. |
| Databáze: | OpenAIRE |
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