Whole-Blood Counting Immunoassay as a Short-Turnaround Test for Detection of Hepatitis B Surface Antigen, Anti-Hepatitis C Virus Antibodies, and Anti-Treponema pallidumAntibodies
| Autor: | Yukiko Nishiyama, Takako Okuda, Toyoichiro Kudo, Aiko Kido, Satoshi Ichiyama, Hiroshi Koganeya, Yoshitsugu Iinuma, Motoshige Nabeshima |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
musculoskeletal diseases
Microbiology (medical) HBsAg Time Factors Biology medicine.disease_cause Sensitivity and Specificity Serology Immunoenzyme Techniques Orthohepadnavirus medicine Humans Treponema pallidum Hepatitis B Antibodies Immunoassay Hepatitis B virus Hepatitis B Surface Antigens medicine.diagnostic_test Reproducibility of Results Bacteriology Hepatitis B biology.organism_classification medicine.disease Antibodies Bacterial Virology Hepadnaviridae biology.protein Antibody |
| Zdroj: | Journal of Clinical Microbiology. 42:4250-4252 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.42.9.4250-4252.2004 |
| Popis: | Whole-blood samples were used for a counting immunoassay (CIA) with the aim of developing a short-turnaround test. After optimization of the CIA, hepatitis B surface antigen (HBsAg), anti-hepatitis C virus antibodies (anti-HCV), and anti-Treponema pallidum antibodies (anti-TP) were detected as efficiently as by an enzyme immunoassay (EIA) with serum samples. The correlations between whole-blood CIA and serum EIA were 99.8, 97.1, and 99.4% for HBsAg, anti-HCV, and anti-TP, respectively. Whole-blood CIA may be of value when rapid screening of many samples is required. The counting immunoassay (CIA) is an application of particle counting technology to serological tests (6). Latex particles are agglutinated by antibodies or antigens of interest and are quantified by scattered laser light while passing through a controlled sheath flow, which is also used in flow cytometry. A similar method is the particle counting immunoassay, in which nonagglutinated particles are counted with the aid of instrumentation (1, 4, 5). Reported applications of these methods, most of which use serum samples, include the detection of hepatitis B virus (HBV) antigens or antibodies (1), anti-adult T-cell leukemia antibodies (6), antitoxoplasma antibodies (2), urinary cotinine (3), hormones (4), and serum acute-phase proteins. The advantages of CIA in comparison with traditional enzymatic methods include a reaction time as short as 15 min, high throughput, and small sample volumes. Taking these advantages into account, we evaluated whole-blood assays by using CIA to develop a short-turnaround test. In general, the preferred sample for a short-turnaround test is whole blood because the preparation of serum samples, including centrifugation time, inevitably takes 30 to 40 min after the blood has been drawn. Since currently available CIA reagents are designed for serum samples, we decided to optimize CIA reagents for whole blood. Whole-blood samples were tested for hepatitis B, hepatitis C, and syphilis, with the reagents, detector, and internal software all optimized. The results were compared with those obtained by an enzyme immunoassay (EIA) with paired serum samples. |
| Databáze: | OpenAIRE |
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