Identification of phenolic metabolites in human urine after the intake of a functional food made from grape extract by a high resolution LTQ-Orbitrap-MS approach

Autor: Gemma Sasot, Miriam Martínez-Huélamo, Rosa M. Lamuela-Raventós, Mercè Mercader-Martí, Ramon Estruch, Anna Vallverdú-Queralt
Přispěvatelé: Nutrition, Food Science and Gastronomy Department, School of Pharmacy and Food Science, University of Barcelona, Biomedical Research Center in Red-Physiopathology of Obesity and Nutrition (CIBERobn), INSA-UB, Nutrition and Food Safety Research Institute, Sciences Pour l'Oenologie (SPO), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Miguel Torres S.A., Department of Internal Medicine, Hospital Clinic, Institute of Biomedical Investigation August Pi i Sunyer (IDIBAPS), INNPRONTA Project [IPT-20111008], CICY [AGL2013-49083-C3-1-R, AGL2016-79113-R], Instituto de Salud Carlos III, ISCIII (CIBEROBN) from Spanish Ministrerio de Economia y Competitividad (MEC) (AEI/FEDER, UE), Generalitat de Catalunya (GC) [2014 SGR 773], Alfonso Martin Escudero Foundation [EA 1517], Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université Montpellier 1 (UM1)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Université Montpellier 1 (UM1)-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Adult
Male
0301 basic medicine
Spectrometry
Mass
Electrospray Ionization
Resolution (mass spectrometry)
polyphenol metabolites
Electrospray ionization
mass spectrum analysis
grape pomace
Urine
Mass spectrometry
01 natural sciences
Young Adult
03 medical and health sciences
polyphénol
Flavonols
Phenols
Functional food
Functional Food
Reference Values
human urine
spectrométrie de masse
Humans
Vitis
Prospective Studies
Chromatography
High Pressure Liquid
LTQ-Orbitrap
polyphenols
chemistry.chemical_classification
marc de raisin
Cross-Over Studies
030109 nutrition & dietetics
Chromatography
Plant Extracts
010401 analytical chemistry
Pomace
0104 chemical sciences
chemistry
Polyphenol
high resolution mass spectrometry
identification
Female
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Food Science
Zdroj: Food Research International
Food Research International, Elsevier, 2017, 100, pp.435-444. ⟨10.1016/j.foodres.2017.01.020⟩
ISSN: 0963-9969
DOI: 10.1016/j.foodres.2017.01.020⟩
Popis: Grape pomace (GP) is known to be a rich source of polyphenols with biological activity which may be used as functional ingredients for the development of new health-promoting products. Numerous studies have reported that bioactive compounds may act through multiple mechanisms. In order to verify the oral absorption and metabolism of grape polyphenols, we performed a prospective, randomised and cross-over acute study in 12 volunteers with two interventions: 500 mL of a functional beverage enriched with 200 mL of GP and 500 mL of a control beverage without GP. In this work, liquid chromatography coupled with an electrospray ionization hybrid linear ion trap quadrupole-Orbitrap-mass spectrometry (LC/ESI-LTQ-Orbitrap-MS) technique has been used to accurately identify phenolics in GP and human urine. In GP, 41 phenolic compounds were identified mainly procyanidins, phenolic acids and flavonols, and in human urine over 70 metabolites of phenolic compounds including microbiota metabolites, glucuronides and sulfate derivatives were detected. Overall, high resolution mass spectrometry (HR-MS) enhances the identification of a large variety of polyphenols and their metabolites with great mass accuracies for all molecular ions.
Databáze: OpenAIRE
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