The role of apoptosis in antibody-dependent cellular cytotoxicity
| Autor: | S. J. Curnow, M. J. Glennie, G. T. Stevenson |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Cancer Research
Programmed cell death Lymphoma Lymphocyte Guinea Pigs Immunology Apoptosis Biology Jurkat cells Antibodies Natural killer cell Mice Tumor Cells Cultured medicine Animals Humans Immunology and Allergy Cytotoxic T cell Lymphocytes Cytotoxicity Antibody-dependent cell-mediated cytotoxicity Mice Inbred BALB C Leukemia Experimental Cell Death Antibody-Dependent Cell Cytotoxicity DNA Neoplasm Chromium Radioisotopes Killer Cells Natural medicine.anatomical_structure Oncology Mice Inbred CBA Cancer research biology.protein Immunotherapy Rabbits Antibody |
| Zdroj: | Cancer Immunology Immunotherapy. 36:149-155 |
| ISSN: | 1432-0851 0340-7004 |
| DOI: | 10.1007/bf01741085 |
| Popis: | Apoptosis in three lymphoma cell lines has been studied following cytotoxicity induced in vitro by normal human blood lymphocytes utilizing either natural killer (NK) or antibody-dependent cellular cytotoxic (ADCC) mechanisms. Guinea-pig L2C leukaemic lymphocytes, but not the human cell lines Daudi and Jurkat, revealed a degree of time- and temperature-dependent apoptotic death upon simple culture in vitro. NK cytotoxicity at low effector:target ratios (E:T) induced both release of 51Cr and apoptosis. However NK cytotoxicity at higher E:T, and ADCC at all E:T, increased the level of 51Cr release while reducing the level of apoptosis. The findings were consistent with the apoptotic process being cut short by intervention of necrotic death. The same characteristics accompanied ADCC whether the effectors were recruited by Fc gamma regions of antibody coating the targets, or by bispecific antibodies attaching one arm to the targets and the other to Fc gamma receptors type III on effectors. This finding, and the high level of cytotoxicity elicited by the bispecific method, confirm the belief that NK cells, in addition to exerting NK cytotoxicity, represent the principal effectors for ADCC among blood mononuclear cells. Our results suggest that NK cells have both apoptotic and necrotic mechanisms available for killing their targets, but use only the latter for ADCC. |
| Databáze: | OpenAIRE |
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