T-Cell Receptor Vβ-Family Usage in Primary Cutaneous and Primary Nodal T-Cell Non-Hodgkin's Lymphomas
| Autor: | Marcel G.J. Tilanus, Henk-Jan Schuurman, Bernard De Geus, Willem A. van Vloten, Roel A. de Weger, Abraham H Preesman, Dick F. van Wichen, H. Z. Hu, Roelie Reitsma |
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| Jazyk: | angličtina |
| Předmět: |
Pathology
medicine.medical_specialty Receptors Antigen T-Cell alpha-beta T cell Molecular Sequence Data Gene Expression Dermatology Biology Lymphoma T-Cell Polymerase Chain Reaction Biochemistry Immunophenotyping hemic and lymphatic diseases Tumor Cells Cultured medicine Humans RNA Messenger Gene Rearrangement beta-Chain T-Cell Antigen Receptor Molecular Biology Lymph node Mycosis fungoides Base Sequence T-cell receptor Cell Biology medicine.disease Immunohistochemistry Molecular biology Peripheral T-cell lymphoma Reverse transcriptase Lymphoma T-Cell Cutaneous medicine.anatomical_structure Monoclonal Primer (molecular biology) |
| Zdroj: | Journal of Investigative Dermatology. (5):587-593 |
| ISSN: | 0022-202X |
| DOI: | 10.1111/1523-1747.ep12667988 |
| Popis: | To evaluate whether the expression of T-cell receptor (TCR) V beta families in eight cases of malignant T-cell lymphomas took place in a preferential manner, we analyzed four cases of mycosis fungoides (MF), the most common form of primary cutaneous T-cell non-Hodgkin's lymphomas (NHL), and four cases of primary nodal T-cell NHL. The usage of V beta families in T-cell populations was investigated on mRNA that was transcribed to cDNA using a C beta primer and reverse transcriptase. Subsequently, the specific usage of the families was analyzed by polymerase chain reaction (PCR) using combinations of the selected C beta-oligonucleotide primer and one of the family-specific V beta primers. Peripheral blood lymphocytes from four healthy volunteers and 1 "reactive" lymph node served as a control and expressed all 20 V beta families tested for. In T-cell lines, with restricted V beta expression, and in three patients with advanced MF, only one or two V beta families were expressed at the mRNA level. In an early MF lesion this monoclonal expression was absent: several V beta families were expressed with a weak intensity. This may indicate either a polyclonal origin of MF, or that too few monoclonal neoplastic cells were present in the tissue specimen. In the four nodal T-cell NHL, only one family could be clearly distinguished, whereas some of the other V beta families showed only a weak expression. These latter families represent the reactive T-cell component in the nodal T-cell NHL. Both in nodal T-cell NHL and in MF there was no preferential expression of a particular V beta family. There was a good correlation between PCR data and the expression of V beta-family protein products observed by immunohistochemistry on tissue sections of the T-cell lymphomas. All T-cell lines, three cases of MF, and three cases of nodal T-cell NHL showed a rearrangement of the TCR beta chain on DNA level. |
| Databáze: | OpenAIRE |
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