Derivation of a new hematopoietic cell line with endothelial features from a patient with transformed myeloproliferative syndrome: a case report
| Autor: | Süleyman Ergün, R.-Peter Henke, Udo Schumacher, Walter Fiedler, Gabi Vohwinkel, Ursula M. Gehling, Dieter K. Hossfeld, Nerbil Kilic |
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| Rok vydání: | 2000 |
| Předmět: |
CD31
Adult Cancer Research Pathology medicine.medical_specialty CD34 Biology Polymerase Chain Reaction Cell Line Mice medicine Tumor Cells Cultured Animals Humans Endothelium Lymph node Thrombocytosis Leukemia Cell Differentiation DNA Neoplasm Endoglin medicine.disease Hematopoietic Stem Cells Immunohistochemistry Endothelial stem cell Haematopoiesis medicine.anatomical_structure Oncology Hemangioblast Female |
| Zdroj: | Cancer. 88(2) |
| ISSN: | 0008-543X |
| Popis: | BACKGROUND During embryonal development primitive hematopoiesis can be observed first in the yolk sac, in which both hematopoietic and endothelial cells are derived from a common precursor, the hemangioblast. Whether cells with this dual differentiation potential persist during postnatal life is unknown. METHODS A cell line was derived from a patient with secondary acute leukemia. Because of its ability to grow in soft agar and in SCID mice, this cell line was analyzed for expression of differentiation antigens by fluorescence-activated cell sorter analysis, immunocytochemistry, fluorescent in situ hybridization (FISH) analysis with simultaneous cell surface staining, and polymerase chain reaction (PCR). RESULTS A new cell line was established from a patient with essential thrombocytosis that transformed into acute leukemia. The patient's initial clinical presentation included skin and lymph node infiltrations that were taken for an angiosarcoma due to positivity for CD34, CD31, and von Willebrand factor on immunohistology. In addition to hematopoietic markers, leukemic cells expressed endothelial antigens such as CD62E, CD105, and bound Ulex europaeus lectin-1. Immunocytochemistry revealed positive staining for vascular endothelial growth factor receptor type 2 (KDR), Tie-2/Tek, the angiopoietin receptor, and vascular endothelial cadherin. These results were confirmed by PCR analysis. Simultaneous staining for CD62E and FISH analysis showed that cells with endothelial characteristics belonged to the leukemia. FISH analysis of histologic sections of the lymph node infiltration confirmed this manifestation as part of the leukemic process. The derived cell line, UKE-1, forms colonies in soft agar and is tumorigenic in SCID mice. CONCLUSIONS This new cell line, UKE-1, appears to combine hematopoietic and endothelial features, indicating the close ontogenic relation of both lineages. Cancer 2000;88:344–51. © 2000 American Cancer Society. |
| Databáze: | OpenAIRE |
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