Metabolic engineering of xanthophyll content in tomato fruits
| Autor: | Riccardo Aquilani, Bilal Camara, Florence Bouvier, Patrizia Pallara, Sridhar Dharmapuri, Carlo Rosati, Giovanni Giuliano |
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| Jazyk: | angličtina |
| Předmět: |
Zeaxanthin
medicine.medical_treatment Transgene Blotting Western Biophysics Arabidopsis Gene Expression β-Carotene Xanthophylls Biochemistry Mixed Function Oxygenases β-Cryptoxanthin Metabolic engineering chemistry.chemical_compound Solanum lycopersicum Structural Biology Zeaxanthins Genetics medicine Transgenes Intramolecular Lyases Molecular Biology Carotenoid Cryptoxanthins Genes Dominant chemistry.chemical_classification biology Reverse Transcriptase Polymerase Chain Reaction Carotene food and beverages Cell Biology Plants Genetically Modified beta Carotene Enzyme assay Lycopene Plant Leaves Phenotype chemistry Xanthophyll Fruit biology.protein Genetic Engineering |
| Zdroj: | FEBS Letters. (1-3):30-34 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/S0014-5793(02)02699-6 |
| Popis: | Ripe tomato fruits accumulate significant amounts of the linear carotene lycopene, but only trace amounts of xanthophylls (oxygenated carotenoids). We overexpressed the lycopene beta-cyclase (b-Lcy) and beta-carotene hydroxylase (b-Chy) genes under the control of the fruit-specific Pds promoter. Transgene and protein expression was followed through semi-quantitative reverse transcription-PCR, Western blotting, and enzyme assays. Fruits of the transformants showed a significant increase of beta-carotene, beta-cryptoxanthin and zeaxanthin. The carotenoid composition of leaves remained unaltered. The transgenes and the phenotype are inherited in a dominant Mendelian fashion. This is the first example of successful metabolic engineering of xanthophyll content in tomato fruits. |
| Databáze: | OpenAIRE |
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