Novel Folate Binding Protein in Arabidopsis Expressed during Salicylic Acid-Induced Folate Accumulation
| Autor: | Bijesh Puthusseri, Mohammed Azharuddin Savanur, Peethambaran Divya, Veeresh Lokesh, Bhagyalakshmi Neelwarne, Gyanendra Kumar |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Arabidopsis Metabolic engineering 03 medical and health sciences chemistry.chemical_compound Folic Acid Plant Growth Regulators Amino Acid Sequence Peptide sequence Messenger RNA biology Arabidopsis Proteins food and beverages General Chemistry biology.organism_classification Folate-binding protein Molecular Weight 030104 developmental biology Biochemistry chemistry Folate receptor Heterologous expression General Agricultural and Biological Sciences Carrier Proteins Salicylic Acid Sequence Alignment Salicylic acid |
| Zdroj: | Journal of agricultural and food chemistry. 66(2) |
| ISSN: | 1520-5118 |
| Popis: | Increasing the quantity of natural folates in plant foods is recently gaining significant interest, owing to their acute deficiencies in various populations. This study observed that foliar salicylic acid treatment enhanced the accumulation of folates in Arabidopsis, which correlated with the increase in a folate binding protein (FBP) and the expression of mRNA of a putative folate binding protein At5G27830. A protein band corresponding to ∼43 kDa was observed after resolving the affinity-purified protein on SDS-PAGE, and the partial amino acid sequence indicated that the protein is indeed At5G27830. Docking studies performed with At5G27830 confirmed specific binding of folic acid to predicted site. Heterologous expression of At5G27830 in the yeast resulted in significant uptake and accumulation of folic acid in cells. This novel study of a plant FBP will be useful for folate metabolic engineering of a wide range of crops. |
| Databáze: | OpenAIRE |
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