Oxygen scavenging, NADH oxidase and metronidazole resistance in Helicobacter pylori
| Autor: | David I. Edwards, Mark A. Smith |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Microbiology (medical)
Cell Count Microbiology Multienzyme Complexes Metronidazole medicine NADH NADPH Oxidoreductases Pharmacology (medical) Pharmacology chemistry.chemical_classification Oxidase test NADPH oxidase Helicobacter pylori biology NADH dehydrogenase NADPH Oxidases Drug Resistance Microbial biology.organism_classification Anti-Bacterial Agents Infectious Diseases Enzyme chemistry Mutation biology.protein Polymorphism Restriction Fragment Length Bacteria Intracellular medicine.drug |
| Zdroj: | Journal of Antimicrobial Chemotherapy. 39:347-353 |
| ISSN: | 1460-2091 0305-7453 |
| Popis: | Failure of triple-therapy regimes to eradicate Helicobacter pylori from the stomach is thought to be due to the occurrence of a metronidazole-resistant bacterial population. Exposure of metronidazole-resistant (MtzR) strains of H. pylori to an anaerobic environment causes the activation of metronidazole and the loss of resistance. Using metronidazole-sensitive (MtzS) clinical isolates, we selected mutants conferring resistance to metronidazole, which were used to investigate the effect of bacterial cell density upon the activation of metronidazole. The addition of metronidazole, at a final concentration of 10 mg/L, to MtzR cultures of a bacterial cell density >1 x 10(6) cfu/mL, caused a loss in viability. No loss in viability, however, occurred upon addition of metronidazole to MtzR cultures of a cell density of |
| Databáze: | OpenAIRE |
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