Filamentous condensation of DNA induced by pegylated poly-L-lysine and transfection efficiency
| Autor: | Doriane Richard, Joelle Ogier, Isabelle Nguyen, Benoît Frisch, Patrick Schultz, Vesna Stanic, Corinne Crucifix, Corinne Baehr, Christine Affolter, Youri Arntz |
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| Přispěvatelé: | Institut Gilbert-Laustriat : Biomolécules, Biotechnologie, Innovation Thérapeutique, Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I, Peney, Maité, Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Polymers and Plastics
Cell Survival Cells Lysine Bioengineering 02 engineering and technology Polyethylene glycol Microscopy Atomic Force Transfection 010402 general chemistry 01 natural sciences Polyethylene Glycols Biomaterials Structure-Activity Relationship chemistry.chemical_compound Chlorocebus aethiops PEG ratio Polymer chemistry Materials Chemistry Animals Polylysine Particle Size DNA [SDV.ETH] Life Sciences [q-bio]/Ethics 021001 nanoscience & nanotechnology Grafting Nanostructures 0104 chemical sciences [SDV.ETH]Life Sciences [q-bio]/Ethics Microscopy Electron chemistry Transmission electron microscopy COS Cells Biophysics Nanometre 0210 nano-technology |
| Zdroj: | Biomacromolecules Biomacromolecules, American Chemical Society, 2008, 9 (7), pp.2048-55. ⟨10.1021/bm800287z⟩ Biomacromolecules, 2008, 9 (7), pp.2048-55. ⟨10.1021/bm800287z⟩ |
| ISSN: | 1525-7797 1526-4602 |
| DOI: | 10.1021/bm800287z⟩ |
| Popis: | International audience; In this paper we propose a detailed analysis of structural and morphological properties of two poly-L-lysine (PLL)-based transfection formulations, PLL/DNA and pegylated PLL (PLL-g-PEG)/DNA, by means of atomic force microscopy (AFM) and transmission electron microscopy (TEM). Comparing PLL-g-PEG/DNA with PLL/DNA polyplexes, we demonstrate that, due to the presence of PEG, the particles differ not only in size, shape, and crystalline structure, but also in transfection efficiency. While PLL condensates DNA in large agglomerates, PLL grafted with polyethylene glycol 2000 can condensate DNA in long filaments with diameters of some nanometers (6-20 nm). These structures are dependent on the grafting ratio and are more efficient than compacted ones, showing that DNA uptake and processing by cell is directly related to physicochemical properties of the polyplexes. |
| Databáze: | OpenAIRE |
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