Plasma membrane profiling reveals upregulation of ABCA1 by infected macrophages leading to restriction of mycobacterial growth
| Autor: | Yuxian Du, Yanjia J. Zhang, Babak Javid, Michael P. Weekes, Robin Antrobus, Duncan L. Smith, Jing Long, Robindra Basu Roy |
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| Přispěvatelé: | Medical Research Council & Department for International Development, National Institute for Health Research, Weekes, Michael [0000-0003-3196-5545], Apollo - University of Cambridge Repository |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Cell education lcsh:QR1-502 ABCA1 Microbiology SILAC lcsh:Microbiology Mycobacterium plasma membrane profiling 03 medical and health sciences Downregulation and upregulation Stable isotope labeling by amino acids in cell culture Extracellular medicine Receptor health care economics and organizations Original Research Gene knockdown 030102 biochemistry & molecular biology biology cholesterol 3. Good health Cell biology 030104 developmental biology medicine.anatomical_structure Cholesterol biology.protein lipids (amino acids peptides and proteins) Plasma membrane profiling Intracellular |
| Zdroj: | Long, J, Roy, R B, Zhang, Y J, Antrobus, R, Du, Y, Smith, D L, Weekes, M P & Javid, B 2016, ' Plasma membrane profiling reveals upregulation of ABCA1 by infected macrophages leading to restriction of mycobacterial growth ', Frontiers in Microbiology, vol. 7, no. JUL, 1086 . https://doi.org/10.3389/fmicb.2016.01086 Frontiers in Microbiology Frontiers in Microbiology, Vol 7 (2016) |
| ISSN: | 1664-302X |
| DOI: | 10.3389/fmicb.2016.01086 |
| Popis: | The plasma membrane represents a critical interface between the internal and extracellular environments, and harbors multiple proteins key receptors and transporters that play important roles in restriction of intracellular infection. We applied plasma membrane profiling (PMP), a technique that combines quantitative mass spectrometry with selective cell surface aminooxy-biotinylation, to Bacille Calmette-Guérin (BCG)-infected THP-1 macrophages. We quantified 559 PM proteins in BCG-infected THP-1 cells. One significantly upregulated cell-surface protein was the cholesterol transporter ABCA1. We showed that ABCA1 was upregulated on the macrophage cell-surface following infection with pathogenic mycobacteria and knockdown of ABCA1 resulted in increased mycobacterial survival within macrophages, suggesting that it may be a novel mycobacterial host-restriction factor. |
| Databáze: | OpenAIRE |
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