Myoblasts and Embryonic Stem Cells Differentially Engraft in a Mouse Model of Genetic Dilated Cardiomyopathy
| Autor: | Gisèle Bonne, Gillian Butler-Browne, Karine Vauchez, Adeline Jacquet, Marc Fiszman, Erica Yada, Cyril Catelain, Nathalie Mougenot, Michel Pucéat, Aurélie Papadopoulos, Jean-Thomas Vilquin, Stéphanie Riveron |
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| Přispěvatelé: | Vilquin, Jean-Thomas, Centre de recherche en Myologie – U974 SU-INSERM, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut de Myologie, Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Génétique, pharmacologie et physiopathologie des maladies cardiovasculaires, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Therapie Cellulaire en Pathologie Cardio-Vasculaire (UMR_S 633), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Cardiomyopathy
Dilated Male Pathology medicine.medical_specialty Cellular differentiation [SDV]Life Sciences [q-bio] Cell- and Tissue-Based Therapy 030204 cardiovascular system & hematology Biology Muscle Development Cell Line Myoblasts 03 medical and health sciences Paracrine signalling Mice 0302 clinical medicine Drug Discovery Genetics medicine Myocyte Animals Molecular Biology Embryonic Stem Cells 030304 developmental biology Pharmacology 0303 health sciences Regeneration (biology) Myocardium Graft Survival Dilated cardiomyopathy Cell Differentiation Anatomy medicine.disease Embryonic stem cell Myocardial Contraction Transplantation [SDV] Life Sciences [q-bio] Disease Models Animal Phenotype Cell culture embryonic structures Molecular Medicine Female Original Article |
| Zdroj: | Molecular Therapy Molecular Therapy, 2013, 21 (5), pp.1064-1075. ⟨10.1038/mt.2013.15⟩ Molecular Therapy; Vol 21 |
| ISSN: | 1525-0016 1525-0024 |
| DOI: | 10.1038/mt.2013.15⟩ |
| Popis: | International audience; The functional and architectural benefits of embryonic stem cells (ESC) and myoblasts (Mb) transplantations into infarcted myocardium have been investigated extensively. Whereas ESC repopulated fibrotic areas and contributed to myocardial regeneration, Mb exerted their effects through paracrine secretions and scar remodeling. This therapeutic perspective, however, has been less explored in the setting of nonischemic dilated cardiomyopathies (DCMs). Our aim was to compare the integration and functional efficacy of ESC committed to cardiac fate by bone morphogenic protein 2 (BMP-2) pretreatment and Mb used as gold standard following their transplantation into the myocardium of a mouse model of laminopathy exhibiting a progressive and lethal DCM. After 4 and 8 weeks of transplantation, stabilization was observed in Mb-transplanted mice (P = 0.008) but not in groups of ESC-transplanted or medium-injected animals, where the left ventricular fractional shortening (LVFS) decreased by 32 ± 8% and 41 ± 8% respectively. Engrafted differentiated cells were consistently detected in myocardia of mice receiving Mb, whereas few or no cells were detected in the hearts of mice receiving ESC, except in two cases where teratomas were formed. These data suggest that committed ESC fail to integrate in DCM where scar tissue is absent to provide the appropriate niche, whereas the functional benefits of Mb transplantation might extend to nonischemic cardiomyopathy. |
| Databáze: | OpenAIRE |
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