Differential inhibition of HIV-1 cell binding and HIV-1-induced syncytium formation by low molecular weight sulphated polysaccharides
| Autor: | Shirley S. Schuffman, William M. Mitchell, J. M. Rowland, A. Modliszewski, W E Robinson, David C. Montefiori |
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| Rok vydání: | 1990 |
| Předmět: |
CD4-Positive T-Lymphocytes
Microbiology (medical) Biology Antiviral Agents Giant Cells Virus Cell Line Glycosaminoglycan chemistry.chemical_compound medicine Humans Pharmacology (medical) Glycosaminoglycans Cytopathic effect Pharmacology Syncytium Molecular mass Dextran Sulfate virus diseases Dextrans RNA-Directed DNA Polymerase In vitro Molecular Weight Infectious Diseases Dextran Biochemistry Mechanism of action chemistry HIV-1 medicine.symptom |
| Zdroj: | Journal of Antimicrobial Chemotherapy. 25:313-318 |
| ISSN: | 1460-2091 0305-7453 |
| DOI: | 10.1093/jac/25.3.313 |
| Popis: | Dextran sulphate (MW 5000 and 8000) and a polysulphated glycosaminoglycan (MW 10,000), at concentrations that provided complete protection in a homologous infection assay, failed to block syncytium formation and the resulting cytopathic effect when MT-2 cells were mixed with H9/HIV-1 cells. These substances also had no antiviral activity when added to cells, after virus challenge, at a time when binding and entry were complete. However, a high molecular weight (500,000) dextran sulphate blocked HIV-1 infection at both stages. Thus, the gp120-CD4 interactions mediating HIV-1 binding and HIV-1-induced syncytium formation are differentially affected by this class of polyanionic substances. Furthermore, size may be a determining factor in their potential application as anti-HIV treatment. |
| Databáze: | OpenAIRE |
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