Prebiotics enhance the biotransformation and bioavailability of ginsenosides in rats by modulating gut microbiota
| Autor: | Jun Chen, An Liu, Shuiming Xiao, Shaojing Li, Jiang Xu, Feipeng Duan, Xiaoyan Zhang, Sha Chen, Wen Zhong, Wei Sheng |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Bioavailability SD Sprague Dawley medicine.medical_treatment Prebiotic Gut flora chemistry.chemical_compound 0302 clinical medicine Biotransformation FOS fructooligosaccharide lcsh:Botany Prevotella IS internal standard ANOVA analysis of variance CK compound K biology Galactooligosaccharide UPLC-ESI-QqQ-MS/MS ultra-high pressure liquid chromatography coupled to an electrospray ionization source and a triple-quadrupole mass spectrometer lcsh:QK1-989 GOS galactooligosaccharide CAT CAZymes Analysis Toolkit Biochemistry Ginsenoside 030220 oncology & carcinogenesis LLOQs lower limits of quantifications NMDS non-metric multidimensional scaling Research Article Tmax time of maximum plasma concentration Biotechnology AUC area under the concentration-time curve FDR false discovery rate LEfSe LDA effect size LCA lowest common ancestor Gut microbiota Biochemistry Genetics and Molecular Biology (miscellaneous) 03 medical and health sciences medicine KEGG the Kyoto Encyclopaedia of Genes and Genomes PCA principal component analysis MANOVA multivariate ANOVA Fructooligosaccharide SRA Sequence Read Archive biology.organism_classification 030104 developmental biology Complementary and alternative medicine chemistry CAZymes carbohydrate active enzymes PCoA principal coordinates analysis Cmax peak plasma concentration LDA linear discriminant analysis MRM multiple reaction monitoring |
| Zdroj: | Journal of Ginseng Research Journal of Ginseng Research, Vol 45, Iss 2, Pp 334-343 (2021) |
| ISSN: | 1226-8453 |
| DOI: | 10.1016/j.jgr.2020.08.001 |
| Popis: | Background: Gut microbiota mainly function in the biotransformation of primary ginsenosides into bioactive metabolites. Herein, we investigated the effects of three prebiotic fibers by targeting gut microbiota on the metabolism of ginsenoside Rb1 in vivo. Methods: Sprague Dawley rats were administered with ginsenoside Rb1 after a two-week prebiotic intervention of fructooligosaccharide, galactooligosaccharide, and fibersol-2, respectively. Pharmacokinetic analysis of ginsenoside Rb1 and its metabolites was performed, whilst the microbial composition and metabolic function of gut microbiota were examined by 16S rRNA gene amplicon and metagenomic shotgun sequencing. Results: The results showed that peak plasma concentration and area under concentration time curve of ginsenoside Rb1 and its intermediate metabolites, ginsenoside Rd, F2, and compound K (CK), in the prebiotic intervention groups were increased at various degrees compared with those in the control group. Gut microbiota dramatically responded to the prebiotic treatment at both taxonomical and functional levels. The abundance of Prevotella, which possesses potential function to hydrolyze ginsenoside Rb1 into CK, was significantly elevated in the three prebiotic groups (P |
| Databáze: | OpenAIRE |
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