Modification of cell wall properties in lettuce improves shelf life
| Autor: | Stephen C. Fry, Mark S. Dixon, Graham J. J. Clarkson, Steve D. Rothwell, Fangzhu Zhang, Carol Wagstaff, Gail Taylor |
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| Rok vydání: | 2010 |
| Předmět: |
Crop and Pasture Production
Cell Membrane Permeability Membrane permeability Food Handling Physiology Plant Biology & Botany XTH Plant Biology Genetically Modified Lactuca Plant Science Genetically modified crops Cell wall Gene Expression Regulation Plant Cell Wall Botany Genetics Leaf size Plant Proteins Anti-sense biology fungi Glycosyltransferases food and beverages Plant Lettuce Plants cell wall properties Xyloglucan endotransglucosylase Plants Genetically Modified biology.organism_classification Research Papers Enzyme assay Genetically modified organism lettuce Gene Expression Regulation biology.protein shelf life XET |
| Zdroj: | Wagstaff, C, Clarkson, G J J, Zhang, F, Rothwell, S D, Fry, S C, Taylor, G & Dixon, M S 2010, ' Modification of cell wall properties in lettuce improves shelf life ', Journal of Experimental Botany, vol. 61, no. 4, pp. 1239-1248 . https://doi.org/10.1093/jxb/erq038 Journal of experimental botany, vol 61, iss 4 Journal of Experimental Botany |
| ISSN: | 1460-2431 0022-0957 |
| DOI: | 10.1093/jxb/erq038 |
| Popis: | It is proposed that post-harvest longevity and appearance of salad crops is closely linked to pre-harvest leaf morphology (cell and leaf size) and biophysical structure (leaf strength). Transgenic lettuce plants (Lactuca sativa cv. Valeria) were produced in which the production of the cell wall-modifying enzyme xyloglucan endotransglucosylase/hydrolase (XTH) was down-regulated by antisense inhibition. Independently transformed lines were shown to have multiple members of the LsXTH gene family down-regulated in mature leaves of 6-week-old plants and during the course of shelf life. Consequently, xyloglucan endotransglucosylase (XET) enzyme activity and action were down-regulated in the cell walls of these leaves and it was established that leaf area and fresh weight were decreased while leaf strength was increased in the transgenic lines. Membrane permeability was reduced towards the end of shelf life in the transgenic lines relative to the controls and bacteria were evident inside the leaves of control plants only. Most importantly, an extended shelf-life of transgenic lines was observed relative to the non-transgenic control plants. These data illustrate the potential for engineering cell wall traits for improving quality and longevity of salad crops using either genetic modification directly, or by using markers associated with XTH genes to inform a commercial breeding programme. |
| Databáze: | OpenAIRE |
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