Removal of Glycosaminoglycans from Bovine Granulosa Cells Contributes to Increased Binding of Hydrogen-3 Heparin
| Autor: | Roy L. Ax, S.K. Boehm, M. E. Bellin, C.M. Stodd |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
endocrine system
Hyaluronoglucosaminidase In Vitro Techniques Glycosaminoglycan chemistry.chemical_compound Hyaluronidase Follicular phase Genetics medicine Animals Binding site Glycosaminoglycans Polysaccharide-Lyases Heparinase Granulosa Cells Heparin Chemistry Heparan sulfate Molecular biology Heparin lyase Chondroitinases and Chondroitin Lyases Heparin Lyase Biochemistry Cattle Female Animal Science and Zoology Food Science medicine.drug |
| Zdroj: | Journal of Dairy Science. 69:531-534 |
| ISSN: | 0022-0302 |
| Popis: | Granulosa cells from small or large bovine follicles were pretreated with enzymes that hydrolyze various glycosaminoglycans, and binding of [3H]-heparin to the granulosa was measured. Binding of [3H] heparin increased significantly after enzymatic pretreatments with chondroitinase ABC and fungal hyaluronidase, and similar results were obtained with granulosa from small and large follicles. No changes in binding of [3H] heparin were detected after hydrolyses with chondroitinase AC and heparinase in either follicle size. Heparitinase, which hydrolyzes heparan sulfate, led to a significant 50% increase in binding of [3H] heparin to granulosa from large follicles but was without effect in small follicles. These results suggest that the lower binding of [3H] heparin, which has been reported with follicular enlargement, may be due to heparan sulfate occupying or obstructing binding sites for heparin on granulosa from large follicles. |
| Databáze: | OpenAIRE |
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