Excessıve fluorıde ıntake alters the MMP-2, TIMP-1 and TGF-β levels of perıodontal soft tıssues: an experımental study ın rabbıts
| Autor: | Mehtap Muglali, Abdurrahman Aksoy, M. Yavuz Gulbahar, Elif Eser Sakallıoğlu, Müge Lütfioğlu, Burcu Baş, Umur Sakallıoğlu |
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| Přispěvatelé: | Ondokuz Mayıs Üniversitesi |
| Rok vydání: | 2010 |
| Předmět: |
Periodontium
Male medicine.medical_specialty Fluorosis Dental Periodontal Ligament Coloring agents Epithelial Attachment Gingiva Dentistry Matrix metalloproteinase Epithelium chemistry.chemical_compound Fluorides TIMP-1 Transforming Growth Factor beta Internal medicine Medicine Animals TGF-beta Coloring Agents General Dentistry Tissue Inhibitor of Metalloproteinase-1 MMP-2 business.industry Soft tissue Excessive fluoride intake Immunohistochemistry Molar Cariostatic Agents Endocrinology chemistry Connective Tissue Matrix Metalloproteinase 2 Rabbits business Fluoride Transforming growth factor |
| Zdroj: | Clinical oral investigations. 16(6) |
| ISSN: | 1436-3771 |
| Popis: | BAS, BURCU/0000-0003-0593-3400; Aksoy, Abdurrahman/0000-0001-9486-312X; GULBAHAR, MUSTAFA YAVUZ/0000-0001-8268-7659; LUTFIOGLU, MUGE/0000-0003-1499-5902; Sakallioglu, Umur/0000-0002-8823-755X WOS: 000311362300008 PubMed: 22143481 Objectives This study evaluated the influence of fluoride on periodontal soft tissues by investigating any alterations in their MMP-2, TIMP-1 and TGF-beta profiles secondary to excessive fluoride intake. Material and methods Fluorosis was induced in 18 rabbits (test group) through consumption of fluoride added to drinking water, whereas 10 rabbits consumed regular tap water as daily supply (control group). Following fluorosis verification, animals were sacrificed and their 1st mandibular molar teeth were utilized in the assessments. MMP-2, TIMP-1 and TGF-beta were separately investigated for gingival epithelium (GE), gingival connective tissue (GC) and periodontal ligament (PL) to evaluate periodontal soft tissues. Histological sections were prepared from the groups, the parameters were determined by immunohistochemistry, and their levels were calculated by quantification of the immunostainings. Results Staining intensity of MMP-2 in GC and PL (p < 0.01); TIMP-1 and TGF-beta of GE, GC and PL (p < 0.01) were higher in the test group compared to those of the control group. Intra-group staining of TIMP-1 was higher than MMP-2 in all test group compartments (p < 0.01) and in the control group GE (p < 0.01). TIMP-1 was also higher than TGF-beta in the GE and PL of the test group (p < 0.05) and in the GE of the control group (p < 0.01). Conclusion These results suggest that excessive fluoride intake may affect periodontal soft tissues by increasing MMP-2, TIMP-1 and TGF-beta, and thereby altering the MMP-2/TIMP-1 and TIMP-1/TGF-beta ratios. Clinical relevance Excessive fluoride consumption may alter the periodontal tissue homeostasis which may be detrimental in the maintenance of periodontal health. Ondokuz Mayis UniversityOndokuz Mayis University The study was supported by the Scientific Research Fund of Ondokuz Mayis University. The authors report no conflict of interest related to this study. |
| Databáze: | OpenAIRE |
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