Differential levels of 'urotensin-II-like' activity determined by radio-receptor and radioimmuno-assays
| Autor: | Diane Naselsky, Brian F. Guida, Stephen A. Douglas, Frederick C. Kull, Jyoti Disa, Nambi Aiyar, David J. Behm, Zhaohui Ao, Jui-Lan Su |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Adult
Male medicine.medical_specialty Physiology Urotensins Vasoactive peptide Radioimmunoassay Urine Biochemistry High-performance liquid chromatography Cellular and Molecular Neuroscience chemistry.chemical_compound Radioligand Assay Endocrinology Urinary levels Reference Values Internal medicine medicine Humans Receptor Chemistry Reproducibility of Results Middle Aged Ut receptor Female Urotensin-II |
| Zdroj: | Peptides. 25(8) |
| ISSN: | 0196-9781 |
| Popis: | Plasma and urinary levels of “urotensin(U)-II-like” substances determined in healthy human volunteers were 12.4 ± 0.6 ng/ml and 2.2 ± 0.3 ng/ml by RIA, an order of magnitude lower than that seen by RRA, 167.5 ± 9.5 ng/ml and 65.2 ± 4.3 ng/ml. HPLC demonstrated the existence of at least three prominent activity peaks in plasma and urine, the more hydrophobic of which did not co-elute with U-II, degradation products or URP. RRA and RIA recognized these peaks with contrasting efficacy. As such, published levels of “U-II-like” activity should be interpreted with caution until a better understanding is obtained regarding what species specific RIA and RRA assay reagents interact with. |
| Databáze: | OpenAIRE |
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