Characterization of gastric cells infection by diverse Helicobacter pylori strains through Fourier-transform infrared spectroscopy
| Autor: | Pedro N. Sampaio, Vanda Marques, Sandra M. Aleixo, Bernardo Cunha, Luís P. Fonseca, Cecília R.C. Calado, Andreia Couto |
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| Rok vydání: | 2019 |
| Předmět: |
Genotype
Virulence 02 engineering and technology Disease Adenocarcinoma Gastric adenocarcinoma cells 010402 general chemistry 01 natural sciences Helicobacter Infections Analytical Chemistry Microbiology Células de adenocarcinoma gástrico Bacterial Proteins Stomach Neoplasms Spectroscopy Fourier Transform Infrared Tumor Cells Cultured medicine Humans Infrared spectroscopy Instrumentation Gene Spectroscopy Antigens Bacterial Helicobacter pylori biology Chemistry Espectroscopia de infravermelho Cancer 021001 nanoscience & nanotechnology medicine.disease biology.organism_classification digestive system diseases Atomic and Molecular Physics and Optics 0104 chemical sciences Biomarcadores 0210 nano-technology Biomarkers Bacteria |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 1386-1425 |
| DOI: | 10.1016/j.saa.2018.11.001 |
| Popis: | The infection of Helicobacter pylori, covering 50% of the world-population, leads to diverse gastric diseases as ulcers and cancer along the life-time of the human host. To promote the discovery of biomarkers of bacterial infection, in the present work, Fourier-transform infrared spectra were acquired from adenocarcinoma gastric cells, incubated with H. pylori strains presenting different genotypes concerning the virulent factors cytotoxin associated gene A and vacuolating cytotoxin A. Defined absorbance ratios were evaluated by diverse methods of statistical inference, according to the fulfillment of the tests assumptions. It was possible to define from the gastric cells, diverse absorbance ratios enabling to discriminate: i) The infection; ii) the bacteria genotype; and iii) the gastric disease of the patients from which the bacteria were isolated. These biomarkers could fasten the knowledge of the complex infection process while promoting a platform for a new diagnostic method, rapid but also specific and sensitive towards the diagnosis of both infection and bacterial virulence. |
| Databáze: | OpenAIRE |
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