Molecular imprinting on PtPd nanoflowers for selective recognition and determination of hydrogen peroxide and glucose
| Autor: | Li Ma, Hai-ying Zhao, Min Liu, Ling Li, Junjia Liu, Cai-ni Fan, Jing Gao |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
biology
Chemistry General Chemical Engineering Molecularly imprinted polymer Substrate (chemistry) 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Catalysis Chemical engineering Transmission electron microscopy biology.protein Precipitation polymerization Glucose oxidase 0210 nano-technology Molecular imprinting Nanogel |
| Zdroj: | RSC advances. 9(58) |
| ISSN: | 2046-2069 |
| Popis: | PtPd nanoflowers (PtPd NFs) exhibit intrinsic peroxidase-like activity as nanozymes, but the nanozymes lack substrate specificity and have low catalytic activity. Herein, a molecularly imprinted nanogel on PtPd NFs was prepared by using 3,3′,5,5′-tetramethylbenzidine (TMB) as the template through the aqueous precipitation polymerization method. After the TMB was washed out, many substrate binding pockets were retained in the PtPd NFs. Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and powder X-ray diffraction (XRD) were employed to characterize the molecularly imprinted polymer (MIP) PtPd nanoflowers (T-MIP-PtPd NFs). The obtained T-MIP-PtPd NFs exhibited enhanced catalytic activity and specific recognition for TMB. Compared with PtPd NFs, T-MIP-PtPd NFs showed a linear range from 0.01–5000 μM and a detection limit of 0.005 μM toward the detection of H2O2. Glucose can also be sensitively detected through cascade reaction by the T-MIP-PtPd NFs and glucose oxidase. Therefore, molecular imprinting on nanozymes technology shows promising application in biocatalysis and sensing fields. |
| Databáze: | OpenAIRE |
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