Folate binding and transport by rat kidney brush-border membrane vesicles
| Autor: | Kenneth E. McMartin, Sudhir K. Joshi, Sneh D. Bhandari |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Male
Kidney Cortex Brush border Glutamine Sodium Biophysics Glutamic Acid chemistry.chemical_element Sodium Chloride Biology Tritium Biochemistry Choline chemistry.chemical_compound Folic Acid Glutamates Electrochemistry Animals Binding site HEPES Microvilli Vesicle Osmolar Concentration Biological Transport Rats Inbred Strains Cell Biology Glutamic acid Hydrogen-Ion Concentration Membrane transport Rats Kinetics Glucose Membrane chemistry |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Biomembranes. 937:211-218 |
| ISSN: | 0005-2736 |
| DOI: | 10.1016/0005-2736(88)90243-x |
| Popis: | [3H]Pteroylglutamic acid (PteGlu) uptake was studied using brush-border membrane vesicles isolated from rat kidney. Results on the uptake of [3H]PteGlu by brush-border membrane vesicles incubated in media of increasing osmolarities demonstrated that uptake was contributed by two components, intravesicular transport and membrane binding. Both the components of the uptake exhibited similar pH dependence, with maxima at pH 5.6, and were found to be saturable mechanisms with Km values of 6.7.10(-7) and 11.2.10(-7) M, respectively. These studies show that PteGlu is transported by isolated rat kidney brush-border membrane vesicles in a manner consistent with a saturable system and that a binding component may be functionally associated with this. |
| Databáze: | OpenAIRE |
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