Evaluation of the Gamma-H2AX Assay for Radiation Biodosimetry in a Swine Model
| Autor: | Mark H. Whitnall, Christophe E. Redon, Daisuke Maeda, Maria Moroni, William M. Bonner |
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| Rok vydání: | 2013 |
| Předmět: |
Male
lymphocytes Pathology Swine immunocytofluorescence-based γ-H2AX Ionizing radiation Histones lcsh:Chemistry Radiation Ionizing Gottingen minipig DNA Breaks Double-Stranded lcsh:QH301-705.5 Cells Cultured Spectroscopy General Medicine Göttingen minipig Total body irradiation Computer Science Applications medicine.anatomical_structure Models Animal Swine Miniature ionizing radiation Whole-Body Irradiation medicine.medical_specialty Cell Survival Biology Article Catalysis Inorganic Chemistry Biodosimetry In vivo fibroblasts medicine Animals Humans Radiosensitivity Physical and Theoretical Chemistry Radiometry Fibroblast Molecular Biology business.industry Organic Chemistry Kinetics lcsh:Biology (General) lcsh:QD1-999 Gamma Rays Nuclear medicine business Ex vivo |
| Zdroj: | International Journal of Molecular Sciences, Vol 14, Iss 7, Pp 14119-14135 (2013) International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 14; Issue 7; Pages: 14119-14135 |
| ISSN: | 1422-0067 |
| Popis: | There is a paucity of large animal models to study both the extent and the health risk of ionizing radiation exposure in humans. One promising candidate for such a model is the minipig. Here, we evaluate the minipig for its potential in γ-H2AX-based biodosimetry after exposure to ionizing radiation using both Cs137 and Co60 sources. γ-H2AX foci were enumerated in blood lymphocytes and normal fibroblasts of human and porcine origin after ex vivo g-ray irradiation. DNA double-strand break repair kinetics in minipig blood lymphocytes and fibroblasts, based on the γ-H2AX assay, were similar to those observed in their human counterparts. To substantiate the similarity observed between the human and minipig we show that minipig fibroblast radiosensitivity was similar to that observed with human fibroblasts. Finally, a strong γ-H2AX induction was observed in blood lymphocytes following minipig total body irradiation. Significant responses were detected 3 days after 1.8 Gy and 1 week after 3.8 and 5 Gy with residual γ-H2AX foci proportional to the initial radiation doses. These findings show that the Gottingen minipig provides a useful in vivo model for validation of γ-H2AX biodosimetry for dose assessment in humans. |
| Databáze: | OpenAIRE |
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