Changes in the Expression of SNAP-25 Protein in the Brain of Juvenile Rats in Two Models of Autism
| Autor: | Jerzy W. Lazarewicz, Jacek Lenart, Elzbieta Zieminska, Ewelina Bratek |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Cerebellum medicine.medical_specialty Synaptosomal-Associated Protein 25 Autism Biology Article 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Internal medicine Gene expression medicine Animals Hippocampus (mythology) Autistic Disorder Valproic Acid Rat models RT-qPCR Snap Brain General Medicine medicine.disease Pathophysiology Rats Thalidomide 030104 developmental biology medicine.anatomical_structure Endocrinology Frontal lobe SNAP-25 Female 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Journal of Molecular Neuroscience |
| ISSN: | 1559-1166 0895-8696 |
| Popis: | The results of genetic studies suggest a possible role for SNAP-25 polymorphism in the development of autism spectrum disorders (ASDs); however, there are no data available on whether changes in SNAP-25 expression also affect animals in rodent models of ASD. The aim of the present study was to explore this issue. The studies included 1-month-old rats representing valproic acid (VPA)- and thalidomide (THAL)-induced models of autism. Their mothers received single doses of VPA (800 mg/kg) or THAL (500 mg/kg) per os on the 11th day of gestation. SNAP-25 protein content in the cerebellum, hippocampus, and frontal lobe was determined using Western blotting, while changes of mRNA levels of Snap25 gene were determined using real-time polymerase chain reaction. Compared to controls, SNAP-25 content was decreased by approximately 35% in all brain structures tested, in both males and females, exclusively in the VPA group. In contrast to this, Snap25 expression, studied in males, was increased in the hippocampus and cerebellum in both, VPA- and THAL-treated rats. We discuss the compliance of these results with the hypothesized role of SNAP-25 in the pathophysiology of ASD and the adequacy of the experimental models used. |
| Databáze: | OpenAIRE |
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