Study on the expression and significance of TGF-β1, p-ERK1/2and K-ras in colorectal cancer using tissue microarray technique
| Autor: | Shouli Wang, Lingling Guo, Yuting Kuang, Ying-ying Wang, Xin Hu, Yu-juan Zhang, Mao-ming Sun |
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| Rok vydání: | 2011 |
| Předmět: |
Tissue microarray
ERK1/2 business.industry Kinase Colorectal cancer Normal colon colorectal cancer lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease lcsh:RC254-282 tissue microarray technique TGF-β1 Immunology Cancer research Extracellular General Earth and Planetary Sciences Medicine Immunohistochemistry Stage (cooking) K-ras business General Environmental Science Transforming growth factor |
| Zdroj: | Cancer Biology & Medicine, Vol 8, Iss 1, Pp 21-26 (2011) |
| ISSN: | 1868-324X 1674-5361 |
| DOI: | 10.1007/s11805-011-0554-z |
| Popis: | OBJECTIVE This study aimed to explore the expression and significance of transforming growth factor β1(TGF-β1), extracellular signal-regulated kinases 1/2 (ERK1/2), and K-ras in colorectal cancer (CRC) using tissue microarray technology.METHODS The expressions of TGF-β1, ERK1/2, and K-ras in colon cancer cells taken from the specimens of 92 CRC patients (stage I: 16 cases, stage II: 28 cases, stage III: 24 cases, and stage IV: 24 cases) were analyzed using tissue microarray technology and immunohistochemistry, and compared with those of 20 normal colon tissue samples.RESULTS High immunoreactive scores (IRS) of TGF-β1, p-ERK1/2, and K-ras protein in CRC were obtained, which were 66.3% (61/92), 59.8% (55/92), and 48.9% (45/92), respectively, and those in normal epithelial cells of colon were 10% (2/20), 20% (4/20), and 30% (6/20), respectively (P0.05).CONCLUSION High level expressions of TGF-β1 and ERK1/2 are closely related to the clinical stages of colon cancer and crosstalk may exist between the 2 signal pathways. |
| Databáze: | OpenAIRE |
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