Molecular analysis of high-level ciprofloxacin resistance in Salmonella enterica serovar Typhi and S. Paratyphi A: need to expand the QRDR region?
| Autor: | S. S. Grover, Malini R. Capoor, Pushpa Aggarwal, R. S. Routela, N. S. Walia, Monorama Deb, P. Bifani, P. K. Pillai, Deepthi Nair |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Adult
DNA Bacterial Male Adolescent Epidemiology medicine.drug_class Antibiotics Drug resistance Salmonella typhi Microbiology Young Adult Minimum inhibitory concentration Plasmid Bacterial Proteins Ciprofloxacin Drug Resistance Bacterial medicine Humans Child Antibacterial agent Base Sequence biology biochemical phenomena metabolism and nutrition biology.organism_classification Enterobacteriaceae Anti-Bacterial Agents Infectious Diseases Salmonella paratyphi A Mutation Female medicine.drug |
| Zdroj: | Epidemiology and Infection. 137:871-878 |
| ISSN: | 1469-4409 0950-2688 |
| DOI: | 10.1017/s0950268808001076 |
| Popis: | SUMMARYFourteen strains of S. Typhi (n=13) and S. Paratyphi A (n=1) resistant to ciprofloxacin were compared with 30 ciprofloxacin decreased-susceptibility strains on the basis of qnr plasmid analysis, and nucleotide substitutions at gyrA, gyrB, parC and parE. In ciprofloxacin-resistant strains, five S. Typhi and a single S. Paratyphi A showed triple mutations in gyrA (Ser83→Phe, Asp87→Asn, Glu133→Gly) and a novel mutation outside the quinolone resistance determining region (QRDR) (Met52→Leu). Novel mutations were also discovered in an isolate (minimum inhibitory concentration 8 μg/ml) in gyrA gene Asp76→Asn and outside the QRDR Leu44→Ile. Out of 30 isolates with reduced susceptibility, single mutation was found in 12 strains only. Genes encoding qnr plasmid (qnr A, qnr B, AAC1-F) were not detected in ciprofloxacin-resistant or decreased-susceptibility strains. Antimicrobial surveillance coupled with molecular analysis of fluoroquinolone resistance is warranted for reconfirming novel and established molecular patterns of resistance, which is quintessential for reappraisal of enteric fever therapeutics. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|