Novel cascade reaction-based fluorescent cyanine chemosensor for cysteine detection and bioimaging in living system
| Autor: | Jianhong Wang, Weihong Zhang, Ying Gan, Jiamin Wang, Linqiang Niu, Cuijuan Zhu, Qijuan Cao, Yang Luo, Muxi Wang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
02 engineering and technology 01 natural sciences Analytical Chemistry Mice chemistry.chemical_compound Cascade reaction In vivo Animals Humans Cysteine Cyanine Homocysteine Fluorescent Dyes Mice Inbred BALB C 010401 analytical chemistry Glutathione 021001 nanoscience & nanotechnology Fluorescence 0104 chemical sciences Spectrometry Fluorescence chemistry Biophysics 0210 nano-technology Intracellular HeLa Cells |
| Zdroj: | Talanta. 219:121291 |
| ISSN: | 0039-9140 |
| DOI: | 10.1016/j.talanta.2020.121291 |
| Popis: | Precise detection of intracellular Cys will be helpful to accurately assess the physiological functions in the physiological and pathological processes. Herein, a new probe Meoeth-Cy-OBz-oCl capable of Cys sensing with high selectivity over other mercaptoamino-acid molecules including Hcy and GSH was developed. The studies on sensing mechanism supported thiols-induced SNAr substitution-rearrangement cascade reaction which allowed discriminating Cys from Hcy/GSH. And its preferential fluorescence response of Meoeth-Cy-OBz-oCl to intracellular Cys was also achieved by means of fluorescence imaging in HeLa cells. Besides, Meoeth-Cy-OBz-oCl was confirmed possessing mitochondria-targeting ability in living cells. In addition, fluorescence imaging in BALB/c mice revealed that Meoeth-Cy-OBz-oCl could visually monitor the variation of Cys in vivo. |
| Databáze: | OpenAIRE |
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