HuB (elavl2) mRNA is restricted to the germ cells by post-transcriptional mechanisms including stabilisation of the message by DAZL
| Autor: | Kirk B. Jensen, B. Kate Dredge, Sophie E. Wiszniak |
|---|---|
| Přispěvatelé: | Wiszniak, Sophie E., Dredge, B. Kate, Jensen, Kirk B. |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Male
Time Factors zebrafish mir-430 family Transcription Genetic Somatic cell RNA Stability Gene Expression translation lcsh:Medicine Regulatory Sequences Nucleic Acid Biochemistry in-vivo DAZL 0302 clinical medicine Molecular Cell Biology humans lcsh:Science 3' Untranslated Regions Zebrafish Regulation of gene expression Genetics 0303 health sciences Multidisciplinary biology Gene Expression Regulation Developmental RNA-Binding Proteins Cell Differentiation Nucleic acids Multidisciplinary Sciences medicine.anatomical_structure ELAV Proteins Organ Specificity Science & Technology - Other Topics Female Germ line development Cellular Types binding proteins Germ cell Research Article Embryonic Development clearance Cell fate determination 03 medical and health sciences component medicine Animals RNA Messenger Biology 030304 developmental biology Germ plasm dead-end lcsh:R Zebrafish Proteins biology.organism_classification gene-expression Germ Cells RNA lcsh:Q 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | PLoS ONE, Vol 6, Iss 6, p e20773 (2011) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The ability of germ cells to carry out a gene regulatory program distinct from the surrounding somatic tissue, and their capacity to specify an entire new organism has made them a focus of many studies that seek to understand how specific regulatory mechanisms, particularly post-transcriptional mechanisms, contribute to cell fate. In zebrafish, germ cells are specified through the inheritance of cytoplasmic determinants, termed the germ plasm, which contains a number of maternal mRNAs and proteins. Investigation of several of these messages has revealed that the restricted localisation of these mRNAs to the germ plasm and subsequent germ cells is due to cis-acting sequence elements present in their 3'UTRs. Here we show that a member of the Hu family of RNA-binding proteins, HuB, is maternally provided in the zebrafish embryo and exhibits germ cell specific expression during embryogenesis. Restriction of HuB mRNA to the germ cells is dependent on a number of sequence elements in its 3'UTR, which act to degrade the mRNA in the soma and stabilise it in the germ cells. In addition, we show that the germ cell specific RNA-binding protein DAZL is able to promote HuB mRNA stability and translation in germ cells, and further demonstrate that these activities require a 30 nucleotide element in the 3'UTR. Our study suggests that DAZL specifically binds the HuB 3'UTR and protects the message from degradation and/or enhances HuB translation, leading to the germ cell specific expression of HuB protein. Refereed/Peer-reviewed |
| Databáze: | OpenAIRE |
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