Effect of high-frequency electromagnetic fields on trophoblastic connexins
| Autor: | Fortunato Vesce, Paola Valbonesi, Carla Biondi, Silvia Franzellitti, Laura Lunghi, Franco Cervellati, Guido Franceschetti, Elena Fabbri |
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| Přispěvatelé: | Cervellati F., Franceschetti G., Lunghi L., Franzellitti S., Valbonesi P., Fabbri E., Biondi C., Vesce F. |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
GENE EXPRESSION
PROTEIN EXPRESSION Cell Survival Blotting Western HUMAN TROPHOBLAST CELLS Connexin Fluorescent Antibody Technique Biology Toxicology Connexins Cell Line Cell membrane Electromagnetic Fields HIGH FREQUENCY ELECTROMAGNETIC FIELDS Microscopy Electron Transmission Gene expression medicine Cyclic AMP Humans RNA Messenger Reverse Transcriptase Polymerase Chain Reaction High-frequency electromagnetic fields Trophoblast Gap Junctions Protein subcellular localization prediction Cell biology Trophoblasts Extravillous trophoblasts Protein Transport medicine.anatomical_structure Membrane protein Ultrastructure Connexin 43 Cyclic AMP Connexins High-frequency electromagnetic fields Extravillous trophoblasts Ultrastructure Intracellular CONNEXIN |
| Popis: | Connexins (Cx) are membrane proteins able to influence trophoblast functions. Here we investigated the effect of high-frequency electromagnetic fields (HF-EMF) on Cx expression and localization in extravillous trophoblast cell line HTR-8/SVneo. We also analysed cell ultrastructural changes induced by HF-EMF exposure. Samples were exposed to pulse-modulated 1817 MHz sinusoidal waves (GSM-217 Hz; 1 h: SAR of 2 W/kg). Cx mRNA expression was assessed through semi-quantitative RT-PCR, protein expression by Western blotting, protein localization by indirect immunoflorescence, cell ultrastructure using electron microscopy. HF-EMF exposure significantly and selectively increased Cx40 and Cx43, without altering protein expression. Nevertheless, Cx40 and Cx43 lost their punctuate fluorescence within the cell membrane, becoming diffuse after HF-EMF exposure. Electron microscopy evidenced a sharp decrease in intercellular gap junction-like structures. This study is the first to indicate that exposure of extravillous trophoblast to GSM-217 Hz signals can modify Cx gene expression, Cx protein localization and cellular ultrastructure. |
| Databáze: | OpenAIRE |
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