Glutaminase-catalyzed γ-glutamylation to produce CCK secretion-stimulatory γ-[Glu]n-Trp peptides superior to tryptophan
| Autor: | Wei Wang, Wensi Zhou, Juan Yang, Dongxiao Sun-Waterhouse, Chun Cui |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Medicine (miscellaneous) Peptide digestive system Calcium in biology Western blotting 03 medical and health sciences 0404 agricultural biotechnology In vitro CaSR TX341-641 Secretion Receptor CAMK Cholecystokinin chemistry.chemical_classification 030109 nutrition & dietetics Nutrition and Dietetics Nutrition. Foods and food supply Glutaminase 04 agricultural and veterinary sciences 040401 food science chemistry Biochemistry γ-[Glu](n=0 1 2 3 4)-Trp Signal transduction Food Science |
| Zdroj: | Journal of Functional Foods, Vol 60, Iss, Pp-(2019) |
| ISSN: | 1756-4646 |
| Popis: | This study follows recent attempts to discover and improve the effectiveness of dietary peptides as stimulators of cholecystokinin (CCK) secretion. This study is the first report about the effect of γ-[Glu](n=1,2,3,4)-Trp, produced via γ-glutamylation by glutaminase, on CCK release and Ca2+-sensing receptor (CaS) activation. All these Trp-containing γ-glutamyl peptides exhibited abilities to trigger CCK secretion at 2.5–10 mM, with the highest amount of CCK (187.19 ± 0.68 pg/mL) occurring at 10 mM of γ-Glu-Trp and 5 mM of [Ca2+]e. A CaSR-mediated Ca2+/CaM/CaMK pathway in γ-[Glu](n=0,1,2,3,4)-Trp-induced CCK secretion was proposed based on the following results: the exposure to γ-Glu-Trp increased the protein expression level (Western bolt analysis); The intracellular calcium ([Ca2+]i) mobilization in response to γ-[Glu](n=0,1,2,3,4)-Trp was strongly enhanced; The inhibitors of signaling pathway proteins (NPS-2143, BAPTA-AM and KN62) abolished partially γ-[Glu](n=1,2,3,4)-Trp-induced CCK secretion. Glutaminase-catalyzed γ-glutamylation might be a useful approach for producing superior CCK secretion peptide stimulators. |
| Databáze: | OpenAIRE |
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