Respiratory Nasal Mucosa in Chronic Rhinosinusitis with Nasal Polyps versus COVID-19: Histopathology, Electron Microscopy Analysis and Assessing of Tissue Interleukin-33
| Autor: | Lia Monica Junie, Maria Aluaș, Lucian Barbu-Tudoran, Monica Muntean, Camelia Albu, Cristina Adela Iuga, Iulian Opincariu, Mihaela Laura Vica, Mihaela D. Lazar, Septimiu Tripon, Horea Matei, Veronica Trombitas, Costel Siserman, Maria Ilieș, Ionuț Isaia Jeican, Silviu Albu, Dan Gheban, Patricia Inișca |
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| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Pathology nasal mucosa nasal polyps business.industry chronic rhinosinusitis interleukin-33 Interleukin COVID-19 Mucous membrane of nose General Medicine Hyperplasia medicine.disease Article Interleukin 33 Immune system otorhinolaryngologic diseases medicine Medicine Histopathology Nasal polyps Respiratory system business |
| Zdroj: | Journal of Clinical Medicine Volume 10 Issue 18 Journal of Clinical Medicine, Vol 10, Iss 4110, p 4110 (2021) |
| ISSN: | 2077-0383 |
| Popis: | (1) Background: Chronic rhinosinusitis with nasal polyps (CRSwNP) is one of the most studied rhinological disorders. Modifications of the respiratory nasal mucosa in COVID-19 patients are so far unknown. This paper presents a comparative morphological characterization of the respiratory nasal mucosa in CRSwNP versus COVID-19 and tissue interleukin (IL)-33 concentration. (2) Methods: We analyzed CRSwNP and COVID-19 samples through histopathology, scanning and transmission electron microscopy and performed proteomic determination of IL-33. (3) Results: Histopathologically, stromal edema (p < 0.0001) and basal membrane thickening (p = 0.0768) were found more frequently in CRSwNP than in COVID-19. Inflammatory infiltrate was mainly eosinophil-dominant in CRSwNP and lymphocyte-dominant in COVID-19 (p = 0.3666). A viral cytopathic effect was identified in COVID-19. Scanning electron microscopy detected biofilms only in CRSwNP, while most COVID-19 samples showed microbial aggregates (p = 0.0148) and immune cells (p = 0.1452). Transmission electron microscopy of CRSwNP samples identified biofilms, mucous cell hyperplasia (p = 0.0011), eosinophils, fibrocytes, mastocytes, and collagen fibers. Extracellular suggestive structures for SARS-CoV-2 and multiple Golgi apparatus in epithelial cells were detected in COVID-19 samples. The tissue IL-33 concentration in CRSwNP (210.0 pg/7 μg total protein) was higher than in COVID-19 (52.77 pg/7 μg total protein) (p < 0.0001), also suggesting a different inflammatory pattern. (4) Conclusions: The inflammatory pattern is different in each of these disorders. Results suggested the presence of nasal dysbiosis in both conditions, which could be a determining factor in CRSwNP and a secondary factor in COVID-19. |
| Databáze: | OpenAIRE |
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