Fragment docking to S100 proteins reveals a wide diversity of weak interaction sites
| Autor: | Anusarka Bhaumik, Yvonne Arendt, Claudio Luchinat, Rebecca Del Conte, Mattia Mori, Marco Porcu |
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| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Models
Molecular Pharmacology Genetics Binding Sites Protein Conformation S100 Proteins Organic Chemistry Plasma protein binding Nuclear magnetic resonance spectroscopy Computational biology Biology Biochemistry law.invention NMR spectra database Protein structure law Docking (molecular) Drug Discovery Molecular Medicine Suppressor Molecule General Pharmacology Toxicology and Pharmaceutics Binding site Nuclear Magnetic Resonance Biomolecular Protein Binding |
| Popis: | The S100 protein family is a highly conserved group of Ca(2+)-binding proteins that belong to the EF-hand type and are considered potential drug targets. In the present study we focused our attention on two members of the family: S100A13 and S100B; the former is involved in the nonclassical protein release of two proangiogenic polypeptides FGF-1 and IL-1alpha that are involved in inflammatory processes, whereas S100B is known to interact with the C-terminal domain of the intracellular tumor suppressor p53 and promote cancer development. We screened, using waterLOGSY NMR experiments, 430 molecules of a generic fragment library and we identified different hits for each protein. The subset of fragments interacting with S100B has very few members in common with the subset interacting with S100A13. From the (15)N-HSQC NMR spectra of the proteins in the presence of those hits the chemical shift differences Deltadelta(HN) were calculated, and the main regions of surface interaction were identified. A relatively large variety of interaction regions for various ligands were identified for the two proteins, including known or suggested protein-protein interaction sites. |
| Databáze: | OpenAIRE |
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