Serodiagnosis and Bacterial Genome of Helicobacter pylori Infection
| Autor: | Susumu Take, Kazuyoshi Gotoh, Osamu Matsushita, Akari Watanabe, Hinako Ojima, Kenji Yokota, Hiroyuki Okada, Aina Ichihara |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Health
Toxicology and Mutagenesis Urea breath test Bacterial genome size Gene mutation Toxicology Genome Microbiology 03 medical and health sciences 0302 clinical medicine Antigen antibody medicine CagA genome biology medicine.diagnostic_test VacA Helicobacter pylori biology.organism_classification 030220 oncology & carcinogenesis biology.protein Medicine 030211 gastroenterology & hepatology Antibody |
| Zdroj: | Toxins Volume 13 Issue 7 Toxins, Vol 13, Iss 467, p 467 (2021) |
| ISSN: | 2072-6651 |
| DOI: | 10.3390/toxins13070467 |
| Popis: | The infection caused by Helicobacter pylori is associated with several diseases, including gastric cancer. Several methods for the diagnosis of H. pylori infection exist, including endoscopy, the urea breath test, and the fecal antigen test, which is the serum antibody titer test that is often used since it is a simple and highly sensitive test. In this context, this study aims to find the association between different antibody reactivities and the organization of bacterial genomes. Next-generation sequences were performed to determine the genome sequences of four strains of antigens with different reactivity. The search was performed on the common genes, with the homology analysis conducted using a genome ring and dot plot analysis. The two antigens of the highly reactive strains showed a high gene homology, and Western blots for CagA and VacA also showed high expression levels of proteins. In the poorly responsive antigen strains, it was found that the inversion occurred around the vacA gene in the genome. The structure of bacterial genomes might contribute to the poor reactivity exhibited by the antibodies of patients. In the future, an accurate serodiagnosis could be performed by using a strain with few gene mutations of the antigen used for the antibody titer test of H. pylori. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |