Experimental Infection of Dogs with Leishmania and Saliva as a Model to Study Canine Visceral Leishmaniasis
| Autor: | Melissa Abbehusen, Manoel Barral-Netto, Maiana Pitombo, Clarissa Teixeira, José Carlos Miranda, Lucilene Amorim, Camila I. de Oliveira, Ana Lucia Abreu-Silva, Dirceu Costa, Aldina Barral, Flávia R.F. Nascimento, Maria do Socorro Pires e Cruz, Kyoshi Fukutani, Rayssa Maria de Araujo Carvalho, Cláudia Brodskyn, Joelma Trigo |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Saliva
Veterinary Microbiology lcsh:Medicine Antibodies Protozoan Parasite load Parasite Load Salivary Glands Dog Diseases Leishmania infantum lcsh:Science Immune Response Multidisciplinary biology Salivary gland Animal Models medicine.anatomical_structure Veterinary Diseases Cytokines Leishmaniasis Visceral Female Immunohistochemical Analysis Veterinary Pathology Research Article Veterinary Medicine Immunology Veterinary Immunology Model Organisms Dogs parasitic diseases medicine Animals Psychodidae Biology lcsh:R Veterinary Virology Leishmania biology.organism_classification medicine.disease Veterinary Parasitology Disease Models Animal Visceral leishmaniasis Immunoglobulin G Immunologic Techniques lcsh:Q Veterinary Science Xenodiagnosis |
| Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 4, p e60535 (2013) |
| ISSN: | 1932-6203 |
| Popis: | BACKGROUND: Canine Visceral Leishmaniasis (CVL) is a zoonotic disease caused by Leishmania infantum, transmitted by the bite of Lutzomyia longipalpis sand flies. Dogs are the main domestic reservoir of the parasite. The establishment of an experimental model that partially reproduces natural infection in dogs is very important to test vaccine candidates, mainly regarding those that use salivary proteins from the vector and new therapeutical approaches. METHODOLOGY/PRINCIPAL FINDINGS: In this report, we describe an experimental infection in dogs, using intradermal injection of Leishmania infantum plus salivary gland homogenate (SGH) of Lutzomyia longipalpis. Thirty-five dogs were infected with 1×10(7) parasites combined with five pairs of Lutzomyia longipalpis salivary glands and followed for 450 days after infection and clinical, immunological and parasitological parameters were evaluated. Two hundred and ten days after infection we observed that 31,4% of dogs did not display detectable levels of anti-Leishmania antibodies but all presented different numbers of parasites in the lymph nodes. Animals with a positive xenodiagnosis had at least 3,35×10(5) parasites in their lymph nodes. An increase of IFN-I³ and IL-10 levels was detected during infection. Twenty two percent of dogs developed symptoms of CVL during infection. CONCLUSION: The infection model described here shows some degree of similarity when compared with naturally infected dogs opening new perspectives for the study of CVL using an experimental model that employs the combination of parasites and sand fly saliva both present during natural transmission. |
| Databáze: | OpenAIRE |
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