Endothelium modulates electrical field stimulation-induced contractions of Chelonoidis carbonaria aortic rings
| Autor: | Edson Antunes, Gilberto De Nucci, André Almeida Schenka, André S. Pupo, Rafael Campos, Fabíola Z. Mónica, Felipe Fernandes Jacintho, Ronilson Agnaldo Moreno, Valéria Barbosa de Souza, José Britto-Júnior, Alberto Fernando Oliveira Justo |
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| Přispěvatelé: | Ceará State University (UECE), Universidade Estadual de Campinas (UNICAMP), Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Contraction (grammar) Endothelium Physiology Phenoxybenzamine Health Toxicology and Mutagenesis Dopamine Tetrodotoxin Toxicology Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Phentolamine Catecholamines Internal medicine medicine.artery medicine Prazosin Animals Aorta Chemistry Cell Biology General Medicine Electric Stimulation Turtles 030104 developmental biology medicine.anatomical_structure Endocrinology RÉPTEIS cardiovascular system Female Idazoxan 030217 neurology & neurosurgery medicine.drug Muscle Contraction |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Made available in DSpace on 2020-12-12T02:02:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-07-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The role of endothelium in the electrical-field stimulation (EFS)-induced contractions of Chelonoidis carbonaria aorta was investigated. Contractions were evaluated in the presence and absence of L-NAME (100 μM), tetrodotoxin (1 μM), phentolamine (10 and 100 μM), phenoxybenzamine (1 and 10 μM), prazosin (100 μM), idazoxan (100 μM), atropine (10 μM), D-tubocurarine (10 μM) or indomethacin (10 μM). EFS-induced contraction was also carried out in endothelium-denuded rings. EFS-induced contraction was investigated by the sandwich assay. Concentration curves to endothelin-1 (0.1–100 nM) and U46619 (0.001–100 μM) were also constructed to calculate both Emax and EC50. EFS at 16 Hz contracted Chelonoidis aorta, which was almost abolished by the endothelium removal. The addition of L-NAME increased the EFS response (2.0 ± 0.4 and 8.3 ± 1.9 mN). In L-NAME treated aortic rings, tetrodotoxin did not change the EFS-response (5.1 ± 1.8 and 4.9 ± 1.7 mN). Indomethacin, atropine and d-tubucurarine also did not affect the EFS-response. Phentolamine at 10 μM did not change the EFS-induced contraction; however, at 100 μM, reduced it (3.9 ± 1 and 1.9 ± 0.3 mN). Prazosin and idazoxan did not change EFS-induced contractions. Phenoxybenzamine at 1 μM reduced by 76% (9.6 ± 3.4 and 2.3 ± 0.8 mN) and at 10 μM by 90% the EFS response. Immunohistochemistry identified tyrosine hydroxylase in the endothelium and brain, whereas S100 protein was found only in brain. In conclusion, endothelium modulates EFS-induced contractions in Chelonoidis aortic rings and this modulation may be due to endothelium-derived catecholamines, possibly dopamine. Superior Institute of Biomedical Sciences Ceará State University (UECE) Faculty of Medical Sciences Department of Pharmacology University of Campinas (UNICAMP) Department of Pharmacology Institute of Biosciences of Botucatu UNESP - São Paulo State University Department of Pharmacology Institute of Biomedical Sciences USP – University of São Paulo Department of Pharmacology Institute of Biosciences of Botucatu UNESP - São Paulo State University FAPESP: 2016/04731-8 FAPESP: 2016/09539-8 FAPESP: 2017/15175-1 FAPESP: 2018/24971-9 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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