Role of adenylate cyclase 1 in retinofugal map development
| Autor: | Eric C. Swindell, Hong Ping Xu, Milan Jamrich, Michael C. Crair, Shigeyoshi Itohara, Onkar S. Dhande, Shivani Bhatt, Anastacia Anishchenko, Marla B. Feller, Justin Elstrott, Takuji Iwasato |
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| Rok vydání: | 2012 |
| Předmět: |
Retinal Ganglion Cells
Superior Colliculi genetic structures Biology Lateral geniculate nucleus Cyclase Article Mice Imaging Three-Dimensional medicine Animals Visual Pathways Mice Knockout Brain Mapping Retina Reverse Transcriptase Polymerase Chain Reaction General Neuroscience Superior colliculus Geniculate Bodies Mice Mutant Strains Retinal waves Electrophysiology Electroporation medicine.anatomical_structure Retinal ganglion cell Retinotopy Neuroscience Adenylyl Cyclases |
| Zdroj: | The Journal of Comparative Neurology. 520:1562-1583 |
| ISSN: | 0021-9967 |
| Popis: | The development of topographic maps of the sensory periphery is sensitive to the disruption of adenylate cyclase 1 (AC1) signaling. AC1 catalyzes the production of cAMP in a Ca2+/calmodulin-dependent manner, and AC1 mutant mice (AC1−/−) have disordered visual and somatotopic maps. However, the broad expression of AC1 in the brain and the promiscuous nature of cAMP signaling have frustrated attempts to determine the underlying mechanism of AC1-dependent map development. In the mammalian visual system, the initial coarse targeting of retinal ganglion cell (RGC) projections to the superior colliculus (SC) and lateral geniculate nucleus (LGN) is guided by molecular cues, and the subsequent refinement of these crude projections occurs via an activity-dependent process that depends on spontaneous retinal waves. Here, we show that AC1−/− mice have normal retinal waves but disrupted map refinement. We demonstrate that AC1 is required for the emergence of dense and focused termination zones and elimination of inaccurately targeted collaterals at the level of individual retinofugal arbors. Conditional deletion of AC1 in the retina recapitulates map defects, indicating that the locus of map disruptions in the SC and dorsal LGN of AC1−/− mice is presynaptic. Finally, map defects in mice without AC1 and disrupted retinal waves (AC1−/−;β2−/− double KO mice) are no worse than those in mice lacking only β2−/−, but loss of AC1 occludes map recovery in β2−/− mice during the second postnatal week. These results suggest that AC1 in RGC axons mediates the development of retinotopy and eye-specific segregation in the SC and dorsal LGN. |
| Databáze: | OpenAIRE |
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