DrosophilaRegulatory factor X is necessary for ciliated sensory neuron differentiation

Autor: Anne Laurençon, Bénédicte Durand, Maurice J. Kernan, Camille Vandaele, Madeleine Coulon-Bublex, Raphaëlle Dubruille, Emiko Shishido, Pierre Couble, Peter Swoboda
Přispěvatelé: Laboratoire de biologie et modélisation de la cellule (LBMC UMR 5239), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Génomique Fonctionnelle de Lyon (IGFL), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon), Karolinska Institutet [Stockholm], Institut NeuroMyoGène (INMG), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Université de Lyon-École normale supérieure - Lyon (ENS Lyon)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Rok vydání: 2002
Předmět:
Male
[SDV]Life Sciences [q-bio]
Genes
Insect
MESH: Genes
Insect
0302 clinical medicine
Morphogenesis
Drosophila Proteins
MESH: Animals
RFX
media_common
0303 health sciences
MESH: Electrophysiology
MESH: Neurons
Afferent
Cilium
Ciliated sensory neuron
Cell Differentiation
MESH: Transcription Factors
MESH: Regulatory Factor X Transcription Factors
Cell biology
DNA-Binding Proteins
Electrophysiology
Drosophila melanogaster
Phenotype
medicine.anatomical_structure
Drosophila
Female
Transduction (physiology)
MESH: Cell Differentiation
MESH: Mutation
MESH: Drosophila Proteins
Recombinant Fusion Proteins
media_common.quotation_subject
Molecular Sequence Data
Regulatory Factor-X
Regulatory Factor X Transcription Factors
Sensory system
Biology
MESH: Phenotype
MESH: Drosophila melanogaster
03 medical and health sciences
MESH: Cilia
Perception
medicine
Animals
Humans
Amino Acid Sequence
Cilia
Neurons
Afferent
Molecular Biology
030304 developmental biology
MESH: Molecular Sequence Data
MESH: Morphogenesis
Sensory neuron
Mutation
MESH: Female
Sequence Alignment
Developmental biology
MESH: DNA-Binding Proteins
030217 neurology & neurosurgery
Transcription Factors
Developmental Biology
Zdroj: Development
Development, Palgrave Macmillan, 2002, 129 (23), pp.5487-5498. ⟨10.1242/dev.00148⟩
Development, 2002, 129 (23), pp.5487-5498. ⟨10.1242/dev.00148⟩
ISSN: 1477-9129
0950-1991
1011-6370
Popis: International audience; Ciliated neurons play an important role in sensory perception in many animals. Modified cilia at dendrite endings serve as sites of sensory signal capture and transduction. We describe Drosophila mutations that affect the transcription factor RFX and genetic rescue experiments that demonstrate its central role in sensory cilium differentiation. Rfx mutant flies show defects in chemosensory and mechanosensory behaviors but have normal phototaxis, consistent with Rfx expression in ciliated sensory neurons and neuronal precursors but not in photoreceptors. The mutant behavioral phenotypes are correlated with abnormal function and structure of neuronal cilia, as shown by the loss of sensory transduction and by defects in ciliary morphology and ultrastructure. These results identify Rfx as an essential regulator of ciliated sensory neuron differentiation in Drosophila.
Databáze: OpenAIRE
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