Inhibition of flower pigmentation by antisense CHS genes: promoter and minimal sequence requirements for the antisense effect
| Autor: | A. R. van der Krol, L. A. Mur, P. P. M. De Lange, J. N. M. Mol, A. R. Stuitje |
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| Přispěvatelé: | VAN DER KROL, Ar, Mur, La, DE LANGE, Pieter, Mol, Jn, Stuitje, Ar |
| Rok vydání: | 1990 |
| Předmět: |
Chalcone synthase
Gene Expression Plant Science Petunia Transcription (biology) Complementary DNA hemic and lymphatic diseases Gene expression chalcone synthase (CHS) gene Genetics skin and connective tissue diseases Promoter Regions Genetic Gene Plant Physiological Phenomena antisense RNA Messenger RNA biology integumentary system Pigmentation fungi food and beverages General Medicine Petunia hybrida Plants biology.organism_classification Molecular biology Antisense RNA Antisense Elements (Genetics) Phenotype biology.protein Genetic Engineering Agronomy and Crop Science Acyltransferases |
| Zdroj: | Plant molecular biology. 14(4) |
| ISSN: | 0167-4412 |
| Popis: | Introduction of a constitutive antisense full-length chalcone synthase (CHS) cDNA gene in petunia can result in an inhibition of flower pigmentation. We have evaluated some of the factors which may be important for the effectiveness of an antisense CHS gene. Antisense CHS genes encoding half-length or quarter-length RNA complementary to the 3′ half of CHS mRNA are able to affect flower pigmentation, while a gene encoding RNA complementary to the 5′ half of CHS mRNA did not show phenotypic effects in transgenic petunia plants. We demonstrate that the RNA encoded by the latter gene has a much lower average steady-state level in leaf tissue than the RNAs encoded by the other antisense gene constructs. We have compared the CaMV 35S and endogenous CHS promoter strengths and intrinsic stabilities of sense and antisense CHS RNAs. From the data we conclude that the constitutive antisense CHS genes are not likely to provide an excess of antisense RNA compared to the CHS mRNA derived from the endogenous genes. Effective inhibition of flower pigmentation is also observed when the antisense CHS gene is under control of the homologous CHS promoter. The results indicate that the mechanism of antisense inhibition cannot solely operate via RNA duplex formation between sense and antisense RNA. © 1990 Kluwer Academic Publishers. |
| Databáze: | OpenAIRE |
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