Revealing thermodynamics of DNA origami folding via affine transformations
| Autor: | Samuel P. Forry, Paul N. Patrone, J. Alexander Liddle, Daniel Schiffels, Jacob M. Majikes, Anthony J. Kearsley, Michael Zwolak |
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| Rok vydání: | 2020 |
| Předmět: |
Systematic error
Calorimetry Differential Scanning Fluorescent reporter Entropy Thermodynamics DNA 02 engineering and technology Biology Nucleic Acid Denaturation 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Nanostructures 0104 chemical sciences Chemical Biology and Nucleic Acid Chemistry DNA nanotechnology Fluorescence Resonance Energy Transfer Genetics Nucleic Acid Conformation DNA origami Affine transformation 0210 nano-technology Fluorescent Dyes |
| Zdroj: | Nucleic Acids Res |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gkaa283 |
| Popis: | Structural DNA nanotechnology, as exemplified by DNA origami, has enabled the design and construction of molecularly-precise objects for a myriad of applications. However, limitations in imaging, and other characterization approaches, make a quantitative understanding of the folding process challenging. Such an understanding is necessary to determine the origins of structural defects, which constrain the practical use of these nanostructures. Here, we combine careful fluorescent reporter design with a novel affine transformation technique that, together, permit the rigorous measurement of folding thermodynamics. This method removes sources of systematic uncertainty and resolves problems with typical background-correction schemes. This in turn allows us to examine entropic corrections associated with folding and potential secondary and tertiary structure of the scaffold. Our approach also highlights the importance of heat-capacity changes during DNA melting. In addition to yielding insight into DNA origami folding, it is well-suited to probing fundamental processes in related self-assembling systems. |
| Databáze: | OpenAIRE |
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